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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of 5'-methylthioribose kinase from Bacillus subtilis and Arabidopsis thaliana.
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Crystallization and preliminary X-ray analysis of 5'-methylthioribose kinase from Bacillus subtilis and Arabidopsis thaliana.

机译：结晶和初步的x射线分析5 ' -methylthioribose激酶从枯草芽孢杆菌和拟南芥。

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摘要

Recombinant Bacillus subtilis 5'-methylthioribose (MTR) kinase has been expressed, purified and subsequently crystallized using the hanging-drop vapor-diffusion technique. With PEG 2000MME as the precipitant, two different crystal forms have been grown in the absence and presence of the detergent CHAPS. These crystals belong to space groups P2(1)2(1)2(1) (unit-cell parameters a = 193.7, b = 83.2, c = 51.6 A) and P2(1)2(1)2 (unit-cell parameters a = 213.8, b = 83.2, c = 51.5 A), respectively. The crystals grown in the presence of CHAPS diffract to 2.2 A resolution at Station X8C, National Synchrotron Light Source (NSLS). For both crystal forms, the presence of two monomers per asymmetric unit is predicted (Matthews coefficient V(M) = 2.29 and 2.52 A(3) Da(-1), respectively). Recombinant C-terminally histidine-tagged Arabidopsis thaliana MTR kinase has also been expressed, purified and refolded into its active form. Rod-shaped crystals of this protein were grown from PEG 8000 using the hanging-drop vapor-diffusion technique. These crystals exhibit the symmetry of space group C2 (unit-cell parameters a = 162.3, b = 83.3, c = 91.0 A, beta = 117.8 degrees ) and diffract to 1.9 A resolution at Station X8C, NSLS. Two monomers are estimated to be present in the asymmetric unit (V(M) = 2.82 A(3) Da(-1)).
机译：重组枯草芽孢杆菌5 ' -methylthioribose(地铁)激酶表达,纯化和随后使用悬滴结晶扩散技术。沉淀剂,两个不同的晶体形式生长在没有和存在洗涤剂皮套裤。组P2 (1) 2 (1) (1) (a =晶胞参数193.7, b = 83.2, c = 51.6)和P2 (1) 2 (1) 2(晶胞参数= 213.8,b = 83.2, c =分别为51.5)。的皮套裤衍射分辨率在2.2站X8C,国家同步光源(NSLS)。两个单体/不对称单位预测(马修斯系数V (M) = 2.29和2.52 (3)Da(1),分别)。histidine-tagged拟南芥地铁激酶也被表达,纯化和复合到它的活性形式。蛋白质从盯住8000使用悬滴扩散技术。晶体展览空间群对称性的C2(晶胞参数= 162.3,b = 83.3, c =91.0,β= 117.8度)和衍射1.9在车站X8C决议,NSLS。单体估计中不对称单元(V (M) = 2.82 (3) Da(1))。

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