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首页> 外文期刊>Anticancer Research: International Journal of Cancer Research and Treatment >The G and F contents in megakaryocyte cell lines after stimulation with phorbol myristate acetate.
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The G and F contents in megakaryocyte cell lines after stimulation with phorbol myristate acetate.

机译：佛波醇肉豆蔻酸酯乙酸盐刺激后巨核细胞系中的G和F含量。

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摘要

Megakaryocyte polyploidization responds to platelet demand and results from the lack of cytoplasmic separation while the nucleus keeps dividing. In order to investigate the role of actin in the megakaryocyte polyploidization, phorbol myristate acetate (PMA, 5 x 10(-9) M), a differentiation marker known to induce megakaryocyte polyploidization, was added to human megakaryocytic cell lines (DAMI and HEL) and G, F and total actins were estimated by DNase I inhibition. After four days of culture in the presence of PMA, G actin contents in pg per 10(6) cells were 13.0 pg +/- 2.8 and 1.0 pg +/- 0.1 for unstimulated DAMI and HEL cells. F actin contents per 10(6) cells were 5.8 pg +/- 1.5 and 0.1 pg +/- 0.0 for DAMI and HEL cells. Addition of PMA for four days to culture significantly increased G actin contents (235% and 268% of controls) and F actin contents (234% and 394%), for DAMI and HEL cell lines, respectively (p < 0.05 by t-test). In contrast, G/F actin ratio was not affected (p < 0.05 by t-test) by PMA. DAMI cells from each ploidy classes were then sorted on an ELITE Coulter and assayed for actin content. While total actin, G actin and F actin per cell increased in polyploid cells cultured with PMA, there was a reduction in G, F and total actin contents per diploid equivalent when cells became polyploid. In conclusion, megakaryocyte polyploidization of these cell lines is not related to an unbalance between G and F actins but would be rather due at least partly to a defect in total actin production that could lead to a prevention of the formation of the constriction ring in telophase.
机译：巨核细胞的多倍体化反应对血小板的需求作出反应，并由于缺乏细胞质分离而导致细胞核不断分裂。为了研究肌动蛋白在巨核细胞多倍体化中的作用，将佛波肉豆蔻酸酯乙酸酯（PMA，5 x 10（-9）M）（一种已知诱导巨核细胞多倍体化的分化标记）添加到人巨核细胞系（DAMI和HEL）中G，F和总肌动蛋白通过DNase I抑制来估算。在PMA存在下培养四天后，对于未刺激的DAMI和HEL细胞，每10（6）个细胞中pg的G肌动蛋白含量为13.0 pg +/- 2.8和1.0 pg +/- 0.1。对于DAMI和HEL细胞，每10（6）个细胞的F肌动蛋白含量为5.8 pg +/- 1.5和0.1 pg +/- 0.0。对于DAMI和HEL细胞系，在培养物中添加四天的PMA分别显着增加了G肌动蛋白含量（对照组的235％和268％）和F肌动蛋白含量（234％和394％）（t检验p <0.05） ）。相反，PMA不会影响G / F肌动蛋白比率（t检验，p <0.05）。然后将每个倍性类别的DAMI细胞在ELITE Coulter上进行分选，并检测肌动蛋白含量。虽然在用PMA培养的多倍体细胞中每个细胞的总肌动蛋白，G肌动蛋白和F肌动蛋白增加，但是当细胞变成多倍体时，每二倍体当量的G，F和总肌动蛋白含量降低。总之，这些细胞系的巨核细胞多倍体化与G和F肌动蛋白之间的不平衡无关，而是至少部分归因于肌动蛋白总产量的缺陷，该缺陷可能导致防止在末期形成收缩环。

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