Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor.

Naba N; Tanaka N; Shiraiwa KKusakabe YFunasaka THaga ANagase HRaz ANakamura KT

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Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor.

机译：结晶和初步的x射线晶体的研究老鼠自分泌能动性的因素。

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Mouse autocrine motility factor (mAMF), a tumour-secreted cytokine that stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour-diffusion method. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 69.97, b = 115.88, c = 73.27 A, beta = 101.76 degrees . There are two subunits (one dimer) per asymmetric unit. Complexes with four-, five- and six-carbon carbohydrate phosphate inhibitors have also been crystallized. The crystals diffract to at least 1.8 A resolution and are suitable for X-ray structure analyses at high resolution.

机译：鼠标自分泌运动因子(mAMF)tumour-secreted刺激细胞的细胞因子迁移体外和体内转移结晶的悬滴vapour-diffusion方法。单斜晶体的空间群P2(1),与单胞参数= 69.97,= 115.88 b, c = 73.27,β= 101.76度。每不对称单位(一个二聚体)。四、五和六元碳水化合物磷酸抑制剂也已结晶。至少1.8的晶体衍射分辨率和适合x射线结构分析了在高分辨率。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第11期|2084-2086|共3页
作者
Naba N; Tanaka N; Shiraiwa KKusakabe YFunasaka THaga ANagase HRaz ANakamura KT;
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作者单位

School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.;

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正文语种英语
中图分类化学;
关键词
Autocrine Systems; experimental mouse; crystalsAutocrine Motility FactorCell LocomotionX-raysLocomotionCrystallization;

机译：自分泌系统;实验鼠标;crystalsAutocrine能动性FactorCellLocomotionX-raysLocomotionCrystallization;

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Crystallization and preliminary X-ray crystallographic studies of mouse autocrine motility factor.

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