Crystallization and preliminary X-ray diffraction analysis of spermidine synthase from Helicobacter pylori.

Lu PK; Chien SY; Tsai JYFong CTLee MJHuang HSun YJ

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of spermidine synthase from Helicobacter pylori.

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Crystallization and preliminary X-ray diffraction analysis of spermidine synthase from Helicobacter pylori.

机译：结晶和初步的x射线衍射分析幽门螺杆菌的亚精胺合成酶幽门。

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Polyamines, such as putrescine, spermidine and spermine, are essential for the regulation of cell proliferation and differentiation in most organisms. Spermidine synthase catalyzes the transfer of the aminopropyl group from decarboxylated S-adenosylmethionine to putrescine in the biosynthesis of spermidine. In this study, spermidine synthase of Helicobacter pylori has been overexpressed in Escherichia coli and purified. Two kinds of spermidine synthase crystals were obtained. One belongs to the monoclinic P2(1) space group, with unit-cell parameters a = 62.78, b = 58.24, c = 74.28 A, beta = 90.9 degrees , and the other belongs to the orthorhombic C222(1) space group, with unit-cell parameters a = 100.43, b = 128.55, c = 143.60 A.

机译：多胺、腐胺、亚精胺和精胺,监管是必不可少的大多数细胞增殖和分化生物。aminopropyl集团的转移脱羧S-adenosylmethionine,腐胺在亚精胺的生物合成。亚精胺合成酶的幽门螺杆菌在大肠杆菌和过表达净化。晶体。单斜P2(1)空间群,与单胞参数= 62.78,= 58.24 b, c = 74.28,β= 90.9度,其他属于的斜方晶系的C222(1)空间群,晶胞参数= 100.43,b = 128.55, c =143.60。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第11期|2067-2069|共3页
作者
Lu PK; Chien SY; Tsai JYFong CTLee MJHuang HSun YJ;
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作者单位

Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 300, Taiwan.;

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正文语种英语
中图分类化学;
关键词
Putrescine; Spermidine Synthase; spermidineX-ray diffraction analysisX-raysS-adenosyl-3-methylthiopropylamineCell Proliferation Regulatory ProcessCrystallization;

机译：腐胺、亚精胺合成酶、spermidineX-ray衍射;

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Crystallization and preliminary X-ray diffraction analysis of spermidine synthase from Helicobacter pylori.

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