Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine.

Yokochi N; Yoshikane Y; Yagi TYamasaki MMikami B

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine.

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Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine.

机译：结晶和初步的x射线分析吡哆醛4-dehydrogenase,第二个酶吡哆醇的降解途径我。

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Pyridoxal 4-dehydrogenase (PLDH; EC 1.1.107) is the second enzyme in the bacterial degradation pathway I of vitamin B(6), which catalyzes the oxidation of pyridoxal to 4-pyridoxolactone using NAD(+). PLDH from Microbacterium luteolum, a dimeric protein with a subunit molecular weight of 38 kDa, was crystallized at 277 K in a drop solution comprising 15%(w/v) polyethylene glycol 4000, 0.15 M sodium acetate, 7.5 mM n-octyl-beta-D-glucoside and 0.075 M Tris-HCl pH 7.5 by the sitting-drop vapour-diffusion method. The crystals were monoclinic and belonged to space group C2, with unit-cell parameters a = 107.0, b = 56.7, c = 130.2 A, beta = 103.6 degrees . Diffraction data were collected from a single crystal to 2.0 A.

机译：吡哆醛4-dehydrogenase (PLDH;第二个酶的细菌降解途径我的维生素B(6)催化吡哆醛氧化4-pyridoxolactone使用NAD(+)。二聚的蛋白质亚基分子量38 kDa的结晶在277 K下降解决方案包括15% (w / v)聚乙二醇4000年,醋酸钠0.15米,7.5毫米n-octyl-beta-D-glucoside和0.075 Tris-HCl pH值7.5 sitting-drop vapour-diffusion方法。晶体是单斜和属于空间群C2,晶胞参数=107.0, b = 56.7, c = 130.2,β= 103.6度。单晶2.0。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第11期|2061-2062|共2页
作者
Yokochi N; Yoshikane Y; Yagi TYamasaki MMikami B;
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Department of Bioresources Science, Faculty of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan.;

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正文语种英语
中图分类化学;
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Pyridoxal; Oxidoreductases; Microbacterium luteolumX-ray analysisPyridoxineDegradation PathwayDrop Solution Dosage Formsodium acetateCrystallization;

机译：吡哆醛;氧化还原酶;小细菌属luteolumX-ray analysisPyridoxineDegradationPathwayDrop溶液用量FormsodiumacetateCrystallization;

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Crystallization and preliminary X-ray analysis of pyridoxal 4-dehydrogenase, the second enzyme in degradation pathway I of pyridoxine.

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