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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization, data collection and phasing of infestin 4, a factor XIIa inhibitor.
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Crystallization, data collection and phasing of infestin 4, a factor XIIa inhibitor.

机译：结晶,数据收集和分阶段的infestin 4, XIIa抑制剂的一个因素。

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摘要

Infestin is a protein from Triatoma infestans (kissing bug) composed of seven Kazal-type domains that is further processed to yield several serine protease inhibitors with varying specificities. Infestins 3 and 4 are the last two domains of the infestin gene and are found in vivo in the insect's anterior midgut. The last domain, infestin 4, has been cloned, expressed and purified. Here, the crystallization of infestin 4 using the sitting-drop vapour-diffusion method with PEG 8000 as precipitant is described. Crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 25.89, b = 45.64, c = 57.41 A. X-ray diffraction data were collected to a maximum resolution of 1.8 A using a synchrotron-radiation source. Initial phases were calculated by molecular replacement using an edited rhodniin molecule as the search model. Structure refinement is in progress.
机译：Infestin是一种蛋白质从Triatoma 5种(接吻虫)七Kazal-type组成域是收益率进一步处理几个丝氨酸蛋白酶抑制剂有不同特异性。域infestin基因和被发现的在昆虫体内的前中肠。域,infestin 4,被克隆,表达和纯化。使用sitting-drop infestin 4vapour-diffusion方法与8000年挂钩沉淀剂。斜方晶系的空间群P2 (1) 2 (1) 2 (1)晶胞参数= 25.89,b = 45.64, c =57.41。1.8使用的最大分辨率电脑同步源。计算了分子替换使用编辑rhodniin分子作为搜索模型。正在进行结构改进。

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