Crystallization and preliminary X-ray crystallographic studies of a lectin from the mushroom Marasmius oreades.

Grahn E; Holmner A; Cronet CTateno HWinter HCGoldstein IJKrengel U

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Crystallization and preliminary X-ray crystallographic studies of a lectin from the mushroom Marasmius oreades.

机译：结晶和初步的x射线晶体的凝集素的研究蘑菇Marasmius山岳女神。

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The Marasmius oreades agglutinin (MOA) recognizes blood group B oligosaccharides. This mushroom lectin belongs to the ricin superfamily and is currently the only lectin known with exclusive specificity for Galalpha1,3Gal-structures, as occur in the subterminally fucosylated blood group B epitope Galalpha1,3(Fucalpha1,2)Galbeta1,4GlcNAc (MOA's preferred ligand) or without fucosylation in the xenotransplantation epitope. MOA has been co-crystallized with the linear blood group B trisaccharide Galalpha1,3Galbeta1,4GlcNAc using the hanging-drop vapour-diffusion technique at room temperature. MOA crystals were grown in the presence of ammonium formate and HEPES buffer. A 3.0 A data set has been collected. Preliminary analysis of the X-ray data is consistent with space group P3(1) or P3(2) and unit-cell parameters a = b = 105, c = 113 A, with two dimers per asymmetric unit.

机译：的Marasmius山岳凝集素(农业部)承认血液B组低聚糖。凝集素属于蓖麻毒素总科和目前唯一已知植物血凝素与排斥特异性Galalpha1, 3 gal-structures,发生在接近端点的focusylated血B组抗原决定基Galalpha1,3 (Fucalpha1,2) Galbeta1,4GlcNAc (MOA吗首选的配体)或没有focusylation异种移植抗原决定基。co-crystallized线性血液B组三糖Galalpha1 3 galbeta1 4 glcnac使用的悬滴vapour-diffusion技术室温。甲酸铵和消息灵通的缓冲区。3.0数据集收集。x射线数据的分析是一致的空间群P3(1)或P3(2)和单胞参数a = b = 105, c = 113,有两个二聚体/不对称单位。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第11期|2038-2039|共2页
作者
Grahn E; Holmner A; Cronet CTateno HWinter HCGoldstein IJKrengel U;
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Department of Chemistry and Bioscience, Chalmers University of Technology, PO Box 462, SE-405 30 Goteborg, Sweden, and Department of Medical Biochemistry, Goteborg University, PO Box 440, SE-405 30 Goteborg, Sweden.;

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正文语种英语
中图分类化学;
关键词
blood group B; Marasmius oreades; fucosylationAgaricalesammonium formateLectinsX-raysCrystallization;

机译：血液B组;Marasmius山岳;focusylationAgaricalesammoniumformateLectinsX-raysCrystallization;

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Crystallization and preliminary X-ray crystallographic studies of a lectin from the mushroom Marasmius oreades.

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