Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I.

Imagawa T; Nakayama H; Katunuma NSakuraba HOhshima TItoh TSako YNomura NTsuge H

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I.

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Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I.

机译：结晶和初步的x射线衍射归巢核酸内切酶分析I-Tsp061I。

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Two crystal forms, rhombohedral and hexagonal, of a homing endonuclease from Thermoproteus sp. IC-061 (I-Tsp0611) were obtained by the hanging-drop and sitting-drop method, respectively. The hexagonal crystals belong to space group P6(3)22, with unit-cell parameters a = b = 111.4, c = 97.6 A, and diffract to 3.2 A resolution on beamline BL44 at SPring-8 (Harima, Japan). The rhombohedral crystals belong to space group R32, with unit-cell parameters a = b = 95.4, c = 192.9 A, and diffract to 2.7 A resolution using a Cu Kalpha rotating-anode generator with an R-AXIS VII detector. The crystal asymmetric unit contained one protein molecule and the solvent contents of the two crystal forms were estimated to be 68.3 and 67.6% by volume, respectively.

机译：两种晶体形式,菱形的和六角形Thermoproteus sp的归巢核酸内切酶。ic - 061 (I-Tsp0611)获得的悬滴和sitting-drop方法,分别。空间群P6(3) 22日,晶胞参数= b = 111.4, c = 97.6, 3.2和衍射决议beamline BL44 SPring-8 (Harima,日本)。集团R32,晶胞参数a = b =95.4、c = 192.9和2.7的衍射解决使用铜Kalpha旋转阳极发电机轴七探测器。水晶不对称单元包含一个蛋白质分子和溶剂的两个内容晶体形式估计为68.3和67.6%分别按体积。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第11期|2006-2008|共3页
作者
Imagawa T; Nakayama H; Katunuma NSakuraba HOhshima TItoh TSako YNomura NTsuge H;
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作者单位

Institute for Health Sciences, Tokushima Bunri University, Yamashiro-cho, Tokushima 770-8514, Japan.;

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正文语种英语
中图分类化学;
关键词
Endonucleases; crystals; HexagonNickaseX-raysX-ray diffraction analysiscrystal outlineCrystallization;

机译：内切酶;晶体;HexagonNickaseX-raysX-ray衍射analysiscrystaloutlineCrystallization;

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Crystallization and preliminary X-ray diffraction analysis of homing endonuclease I-Tsp061I.

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