Crystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosis.

Dias MV; Ely F; Canduri FPereira JHFrazzon JBasso LAPalma MSde Azevedo WF JrSantos DS

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Crystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosis.

机译：结晶和初步的x射线晶体chorismate合成酶的分析结核分枝杆菌。

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The enzymes of the shikimate pathway are potential targets for the development of new therapies because they are essential for bacteria but absent from mammals. The last step in this pathway is performed by chorismate synthase (CS), which catalyzes the conversion of 5-enolpyruvylshikimate-3-phosphate to chorismate. Optimization of crystallization trials allowed the crystallization of homogeneous recombinant CS from Mycobacterium tuberculosis (MtCS). The crystals of MtCS belong to space group P6(4)22 (or P6(2)22) and diffract to 2.8 A resolution, with unit-cell parameters a = b = 129.7, c = 156.8 A. There are two molecules in the asymmetric unit. Molecular-replacement trials were not sucessful. Heavy-atom derivative screening is in progress.

机译：shikimate途径酶的潜力新疗法的发展目标因为他们对于细菌但至关重要缺席的哺乳动物。路径是由chorismate合成酶(CS),催化转化的5-enolpyruvylshikimate-3-phosphate chorismate。优化结晶试验允许的均质重组CS的结晶结核分枝杆菌(mtc)。晶体的矿渣mtc属于空间群P6 (4) 22(或P6(2) 22)和衍射分辨率2.8,晶胞参数a = b = 129.7, c =156.8。不对称单元。没有成功。检查正在进行中。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第11期|2003-2005|共3页
作者
Dias MV; Ely F; Canduri FPereira JHFrazzon JBasso LAPalma MSde Azevedo WF JrSantos DS;
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Departamento de Fisica, UNESP, Sao Jose do Rio Preto, SP 15054-000, Brazil.;

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正文语种英语
中图分类化学;
关键词
shikimate pathway; Mycobacterium tuberculosis; chorismate synthaseX-raysCrystallization;

机译：分枝杆菌shikimate通路;肺结核;chorismatesynthaseX-raysCrystallization;

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Crystallization and preliminary X-ray crystallographic analysis of chorismate synthase from Mycobacterium tuberculosis.

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