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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Production and preliminary analysis of perdeuterated yeast inorganic pyrophosphatase crystals suitable for neutron diffraction
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Production and preliminary analysis of perdeuterated yeast inorganic pyrophosphatase crystals suitable for neutron diffraction

机译：生产和初步分析perdeuterated酵母无机焦磷酸酶适合中子衍射晶体

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摘要

Yeast inorganic pyrophosphatase (Y-PPase) is a model system for studying phosphoryl-transfer reactions catalysed by multiple metal ions. To understand the process requires knowledge of the positions of the protons in the active site, which can be best achieved by neutron diffraction analysis. In order to reduce the hydrogen incoherent-scattering background and to improve the signal-to-noise ratio of the neutron reflections, deuterated protein was produced. Deuterated protein 96% enriched with deuterium was produced in high yield and crystals as large as 2 mm on one side were obtained. These crystals have unit-cell parameters a=58.9, b=103.9, c=117.0 Angstrom, alpha=beta=gamma=90degrees at 273 K and diffract neutrons to resolutions of 2.5-3 Angstrom. The X-ray structure of the perdeuterated protein has also been refined at 273 K to 1.9 Angstrom resolution.
机译：酵母无机焦磷酸酶(Y-PPase)研究phosphoryl-transfer的模型系统反应催化了多种金属离子。理解这个过程需要的知识位置的质子在活性部位,这可以通过中子衍射最好分析。非相干散射背景和改善中子的信噪比产生了反射,使氘化蛋白质。富含氘氘蛋白质96%产自高收益和水晶一样大吗2毫米的一侧。晶胞参数= 58.9,b = 103.9,c = 117.0埃,α=β=γ= 90度273 K和中子衍射的决议2.5 - 3埃。perdeuterated蛋白质也被改进273 K为1.9埃分辨率。

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