Microseed matrix screening to improve crystals of yeast cytosine deaminase

Ireton GC; Stoddard BL

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Microseed matrix screening to improve crystals of yeast cytosine deaminase

机译：Microseed矩阵提高晶体的筛查酵母胞嘧啶脱氨酶

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A crystallization strategy termed 'microseed matrix screening' is described where the optimal conditions for nucleation versus extended lattice growth are not compatible. This method is an extension of conventional seeding techniques in which microseeds from the nucleation step are systematically seeded into new conditions where all components of the drop are allowed to vary to screen for subsequent growth of well ordered specimens. The structure of a crystal form of yeast cytosine deaminase produced by streak-seeding using a single condition for both nucleation and growth is compared with the structure of a related crystal form produced by separating nucleation and growth conditions. The resulting structural comparison demonstrates that differential chelation patterns of cations by acidic surface residues of proteins within crystal lattice contacts is a critical parameter of crystal nucleation and growth.

机译：一个结晶的策略称为“microseed最优矩阵筛选的描述条件的成核和扩展晶格增长不兼容。扩展传统播种技术从成核microseeds一步吗系统地播种到新的情况所有组件允许下降的变化屏幕为随后的增长的命令标本。酵母胞嘧啶脱氨酶产生的streak-seeding使用单一条件成核和生长相比一个相关的晶体结构形式所产生的分离成核和生长的条件。结果表明结构比较微分的阳离子螯合模式酸性表面残留的蛋白质晶格的联系人是一个关键参数晶体的成核和增长。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2004年第3期|601-605|共5页
作者
Ireton GC; Stoddard BL;
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作者单位

Univ Washington, Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Yeast; NUCLEAT; crystalscritical parametercrystal outlineCrystal nucleationCytosine Deaminase;

机译：酵母;NUCLEAT crystalscritical parametercrystaloutlineCrystal nucleationCytosine脱氨酶;

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2. Migration to gliomas of bone mesenchymal stem cells transfected with cytosine deaminase gene following transplantation in vivo [J] . Guangchun Ji, Fei Song, Qi Xing, 中国神经再生研究：英文版 . 2011,第019期
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5. An automated microseed matrix-screening method for protein crystallization [J] . DArcy A, Frederic VA, Marsh M Acta crystallographica.Section D. Biological crystallography . 2007,第Pta4期

机译：一个自动microseed matrix-screening方法蛋白质结晶
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机译：PROPOSED STRATEGIES FOR IMPROVING THE STABILITY OF POLYMERIC HPMC MATRIX TABLETS: PROPER SELECTION OF EXCIPIENTS AND SEAL COATING
7. Genome-wide mapping of regions preferentially targeted by the human DNA-cytosine deaminase APOBEC3A using uracil-DNA pulldown and sequencing [O] . Ramin Sakhtemani, Vimukthi Senevirathne, Jessica Stewart, 2019

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Microseed matrix screening to improve crystals of yeast cytosine deaminase

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