Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a : K30

Beis K; Nesper J; Whitfield CNaismith JH

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Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a : K30

机译：结晶和初步的x射线衍射分析Wza外膜脂蛋白大肠杆菌血清型O9a: K30

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A novel integral membrane lipoprotein, Wza, from Escherichia coli serotype O9a:K30 has been purified and crystallized. Wza is required for the surface expression of the serotype K30 group 1 capsular polysaccharide of E. coli; closely related homologues are found in other bacteria that produce extracellular polysaccharides. The Wza crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a=94.6, b=215.5, c=218.5 Angstrom. A data set to 3.0 Angstrom with 99.8% completeness and an R-merge of 10.5% has been collected from a single crystal.

机译：Wza,从小说整体膜脂蛋白大肠杆菌血清型O9a: K30纯化和结晶。血清型的表面表达K30组1大肠杆菌荚膜多糖;相关的同系物中发现其他细菌产生胞外多糖。Wza晶体属于斜方晶系的空间集团P2(1) 2(1) 2(1),与晶胞参数b = 215.5 = 94.6, c = 218.5埃。3.0埃,完整性和一个99.8%已经收集了从单一R-merge 10.5%晶体。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第3期|558-560|共3页
作者
Beis K; Nesper J; Whitfield CNaismith JH;
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作者单位

Univ St Andrews, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland;

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正文语种英语
中图分类化学;
关键词
Escherichia coli; Escherichia coli serotype; AngstromX-ray diffraction analysisSurfaceslipoproteinsPolysaccharidesCrystallization;

机译：大肠杆菌,大肠杆菌血清型;AngstromX-ray衍射;

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Crystallization and preliminary X-ray diffraction analysis of Wza outer-membrane lipoprotein from Escherichia coli serotype O9a : K30

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