Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis

Sansen S; De Raner CJ; Gebruers KBrijs KCourtin CMDelcour JARabijns A

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Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis

机译：结晶和初步的x射线衍射研究两种复合物TAXI-type木聚糖酶抑制剂与糖苷水解酶家族11木聚糖酶从黑曲霉和芽孢杆菌细小

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Endo-beta-1,4-xylanases hydrolyze arabinoxylan, a major constituent of cereal cell walls, and are nowadays widely used in biotechnological processes. Purified complexes of family 11 xylanases from Aspergillus niger and Bacillus subtilis with TAXI I, a TAXI-type xylanase inhibitor from Triticum aestivum L., were prepared. In both cases the complex was crystallized using the hanging-drop vapour-diffusion method. The needle-like crystals of TAXI I in complex with A. niger xylanase belong to the trigonal space group P3(1) or P3(2), with unit-cell parameters a=b=88.43, c=128.99 Angstrom, and diffract to 1.8 Angstrom resolution. TAXI I in complex with B. subtilis xylanase crystallizes in the monoclinic space group C2, with a=107.89, b=95.33, c=66.31 Angstrom, beta=122.24degrees. Complete data sets were collected for both crystal types using synchrotron radiation.

机译：Endo-beta-1 4-xylanases水解阿糖基木聚糖,谷物细胞壁的主要成分,是现在广泛应用于生物技术流程。木聚糖酶从黑曲霉和芽孢杆菌与出租车我细小,TAXI-type木聚糖酶从小麦L。抑制剂,准备。使用悬滴结晶vapour-diffusion方法。出租车我在复杂的尼日尔木聚糖酶属于三方晶系的空间群P3 (1)P3(2),晶胞参数a = b = 88.43,c = 128.99埃,衍射到1.8埃决议。木聚糖酶在单斜结晶空间C2组,b = 107.89, = 95.33, c = 66.31埃,β= 122.24度。收集两个晶体类型使用同步加速器辐射。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第3期|555-557|共3页
作者
Sansen S; De Raner CJ; Gebruers KBrijs KCourtin CMDelcour JARabijns A;
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Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Analyt Chem & Med Fysicochem, B-3000 Louvain, Belgium;

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正文语种英语
中图分类化学;
关键词
Bacillus subtilis; Purification; BarleyAspergillus nigerAngstromXylan Endo-1,3-beta-XylosidaseCell wallCrystallization;

机译：枯草芽孢杆菌;净化;BarleyAspergillusnigerAngstromXylan Endo-1,3-beta-XylosidaseCellwallCrystallization;

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Crystallization and preliminary X-ray diffraction study of two complexes of a TAXI-type xylanase inhibitor with glycoside hydrolase family 11 xylanases from Aspergillus niger and Bacillus subtilis

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