Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus

Verhaest M; Van den Ende W; Yoshida MLe Roy KPeeraer YSansen SDe Ranter CJVan Laere ARabijns A

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Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus

机译：结晶和初步的x射线衍射果聚糖的研究1-exohydrolase花絮Cichorium intybus

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Fructan 1-exohydrolase IIa (1-FEH IIa), a plant enzyme involved in fructan breakdown, has been crystallized using the hanging-drop vapour-diffusion method at 277 K. The crystals are tetragonal, belonging to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a=139.83, b=139.83, c=181.94 Angstrom. Calculation of the Matthews coefficient indicates there to be two or three molecules in the asymmetric unit. Synchrotron radiation was used to collect a complete native data set to a resolution of 2.35 Angstrom.

机译：果聚糖1-exohydrolase花絮(1-FEH花絮”),一个工厂酶参与果聚糖分解,使用悬滴结晶在277 K vapour-diffusion方法。是正方,属于空间群P4(1) 2(1) 2或P4(3) 2(1) 2,单胞参数= 139.83,= 139.83 b, c = 181.94埃。马修斯的计算系数表示有两个或三个分子不对称单元。收集完整的本地数据设置2.35埃分辨率。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第3期|553-554|共2页
作者
Verhaest M; Van den Ende W; Yoshida MLe Roy KPeeraer YSansen SDe Ranter CJVan Laere ARabijns A;
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作者单位

Katholieke Univ Leuven, Fac Farmaceut Wetenschappen, Lab Analyt Chem & Med Fysicochem, B-3000 Louvain, Belgium;

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正文语种英语
中图分类化学;
关键词
Purification; Cichorium intybus; Plant enzyme, NOSAngstromMolecular cloningCrystallization;

机译：净化;Cichorium intybus;植物酶,NOSAngstromMolecular cloningCrystallization;

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Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybus

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