Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin

Deng L; Markova SV; Vysotski ESLiu ZJLee JRose JWang BC

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin

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Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin

机译：准备和x射线晶体的分析Ca2 +出院就是obelin

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Ca2+-regulated photoproteins belong to the EF-hand Ca2+-binding protein family. The addition of calcium ions initiates bright blue bioluminescence of the photoproteins, a result of the oxidative breakdown of coelenterazine peroxide to coelenteramide. Crystals of the Ca2+-discharged W92F mutant of obelin from Obelia longissima have been grown, representing the first crystallization of a photoprotein after the Ca2+-triggered bioluminescence. A green fluorescence observed from the crystals clearly demonstrates that coelenteramide, the bioluminescence product of coelenterazine peroxide, is bound within the protein. The diffraction pattern exhibits tetragonal Laue symmetry. Systematic absences indicate that the space group is either P4(3)2(1)2 or P4(1)2(1)2. The unit-cell parameters are a=b=53.4, c=144.0 Angstrom. The crystals diffract to 1.9 Angstrom resolution.

机译：Ca2 +调节发光蛋白属于这类ef - hand钙离子结合蛋白家族。钙离子发出明亮的蓝色生物荧光的发光蛋白的结果coelenterazine的氧化分解coelenteramide过氧化。Ca2 +出院W92F突变obelin Obelia对已经成长,代表第一次结晶后的一个例子Ca2 +——生物发光。晶体的荧光观察清楚表明coelenteramide,生物荧光的产物coelenterazine过氧化,势必在蛋白质。衍射模式展品正方劳厄对称。空间群是P4(3) 2(1) 2或P4(1) 2(1) 2。晶胞参数a = b = 53.4, c = 144.0埃。决议。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第3期|512-514|共3页
作者
Deng L; Markova SV; Vysotski ESLiu ZJLee JRose JWang BC;
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作者单位

Univ Georgia, Dept Biochem & Mol Biol, Athens, GA 30602 USA;

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正文语种英语
中图分类化学;
关键词
Luminescent Proteins; Sequences; crystalsBioluminescenceProteinComplementary DNAAequorincoelenterazineCalcium CationCloningobelin;

机译：发光的蛋白质序列;crystalsBioluminesc;

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Preparation and X-ray crystallographic analysis of the Ca2+-discharged photoprotein obelin

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