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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The structure and refinement of apocrustacyanin C-2 to 1.3 angstrom resolution and the search for differences between this protein and the homologous apoproteins A(1) and C-1
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The structure and refinement of apocrustacyanin C-2 to 1.3 angstrom resolution and the search for differences between this protein and the homologous apoproteins A(1) and C-1

机译：apocrustacyanin的结构和细化c - 2到1.3埃分辨率和寻找这种蛋白质和之间的区别同源载脂蛋白(1)和颈- 1

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摘要

The blue carotenoprotein alpha-crustacyanin of Homarus gammarus lobster carapace is comprised chemically of five 20 kDa subunits. Only two genes for the proteins have been isolated (J.B.C. Findlay, personal communication) and the five apoproteins fall into two sets of homologous proteins based on their chemical properties (CRTC, consisting of apoproteins C-1, C-2 and A(1), and CRTA, consisting of apoproteins A(2) and A(3)). The diffraction quality of apo C-2 has been improved from 2.2 to 1.3 Angstrom and its structure solved. The structure is compared with the A(1) and C-1 proteins determined at 1.4 Angstrom [Cianci et al. (2001), Acta Cryst. D57, 1219-1229] and 1.15 Angstrom, respectively [Gordon et al. (2001), Acta Cryst. D57, 1230-1237] and found to be very similar. Normalized B-factor difference plots per residue of different types were used to try to find chemically modified residues; none were found at these resolutions. It remains possible that the differences between the CRTC proteins result from differences in amidation. By comparison of a crystal grown with glycerol (studied at 1.6 Angstrom) and one grown without glycerol (studied at 1.3 Angstrom) it was seen that glycerol bound at the astaxanthin site.
机译：蓝色carotenoprotein alpha-crustacyanin螯龙虾gammarus龙虾甲壳由化学五20 kDa的子单元。基因的蛋白质被隔离(J.B.C.个人沟通的芬德雷市)和五个载脂蛋白分为两组同源根据其化学性质的蛋白质(CRTC颈- 1组成的载脂蛋白、c - 2(1),和CRTA组成的载脂蛋白(2)和(3))。从2.2到1.3埃及其改进结构解决。(1)和颈- 1蛋白确定为1.41219 - 1229年)和1.15埃,分别1230 - 1237年),发现非常相似。规范化b因子不同情节/残留不同类型被用来试图找到化学改性残留物;这些决议。CRTC蛋白质的结果之间的差异酰胺化的差异。晶体生长与甘油(就读于1.6Angstrom)和一个成长没有甘油(研究在1.3埃)看到,甘油虾青素的网站。

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