Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase.

Charron C; Roy H; Blaise MGiege RKern D

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Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase.

机译：结晶和初步的x射线衍射数据的一个古天冬酰胺合成酶相关asparaginyl-tRNA合成酶。

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The archaebacterial-type asparagine synthetase A from Pyrococcus abyssi (AS-AR), which is related to asparaginyl-tRNA synthetase, was crystallized in two different conditions using the hanging-drop vapour-diffusion method. Crystals belonging to space group C2 with unit-cell parameters a = 103.6, b = 43.3, c = 121.5 A, beta = 112.6 degrees and one dimer per asymmetric unit were obtained in the presence of 2-propanol and PEG 4000 at pH 5.6. Another crystal form was obtained in the presence of dioxan and belongs to the monoclinic space group P2(1), with unit-cell parameters a = 96.8, b = 103.9, c = 98.4 A, beta = 107.5 degrees and two dimers per asymmetric unit. Two different native diffraction data sets were collected to 2.3 and 3.0 A resolution using synchrotron radiation and cryocooling for crystals belonging to space groups C2 and P2(1), respectively.

机译：archaebacterial-type的天冬酰胺合成酶从海床abyssi(随着ar1339),这是相关的asparaginyl-tRNA合成酶,结晶在两个不同的使用条件悬滴vapour-diffusion方法。属于空间群与单胞C2参数= 103.6,= 43.3 b, c = 121.5,β= 112.6度,每个不对称单位一个二聚体在丙胺和得到吗4000年在pH值5.6挂钩。二恶烷的存在和属于获得单斜晶体的空间群P2(1),与单胞参数= 96.8,= 103.9 b, c = 98.4,β= 107.5度和两个二聚体/不对称单位。收集2.3和3.0分辨率使用吗同步加速器辐射和cryocooling水晶属于空间组织C2和P2 (1),分别。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第4期|767-769|共3页
作者
Charron C; Roy H; Blaise MGiege RKern D;
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Departement 'Mecanismes et Macromolecules de la Synthese Proteique et Cristallogenese', UPR 9002, Institut de Biologie Moleculaire et Cellulaire du CNRS, 15 Rue Rene Descartes, F--67084 Strasbourg CEDEX, France.;

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正文语种英语
中图分类化学;
关键词
Aspartate-Ammonia Ligase; 1,4-dioxane; Pyrococcus abyssiAmino Acyl-tRNA Synthetasesspace groupsX-ray diffraction dataX-rayscrystal outlineasparaginyl-tRNA synthetaseAsparagineTransfer RNACrystallization;

机译：Aspartate-Ammonia连接酶,1,4 -二恶烷;海床groupsX-ray衍射dataX-rayscrystalsynthetaseAsparagineTransfer RNACrystallization;

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Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase.

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