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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus.
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Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus.

机译：结晶和初步的晶体分析2-keto-3-deoxygluconate激酶

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摘要

2-Keto-3-deoxygluconate kinase (KDGK) catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to 2-keto-3-deoxy-6-phosphogluconate. Two crystal forms of KDGK from Thermus thermophilus were obtained by vapour-diffusion and microbatch methods. Crystals in the form of triangular plates (TtKDGK-1) were obtained that belong to space group P3, with unit-cell parameters a = b = 145.83, c = 74.63 A, and diffract to 3.2 A. These crystals exhibited nearly perfect hemihedral twinning. Assigning six subunits of TtKDGK to the asymmetric unit of the crystal corresponds to a 46.2% solvent content. A single plate-like crystal (TtKDGK-2) belonged to space group P6(3), with unit-cell parameters a = b = 84.83, c = 168.49 A, and diffracts to 2.25 A. This crystal exhibits only partial hemihedral twinning, with a twin fraction of 24.4%. Diffraction-quality crystals of TtKDGK with bound ATP (TtKDGK-ATP), a = b = 84.72, c = 321.61 A and with bound KDG plus the ATP analogue AMP-PNP (TtKDGK-ATP-KDG), with unit-cell parameters a = b = 84.32, c = 168.7 A, were also prepared and characterized.
机译：2-Keto-3-deoxygluconate激酶(KDGK)催化的磷酸化2-keto-3-deoxygluconate2-keto-3-deoxy-6-phosphogluconate (KDG)。晶体的形式从栖热菌属KDGK酸奶通过vapour-diffusion和microbatch吗方法。盘子(TtKDGK-1)获得,属于空间群P3,晶胞参数a = b =145.83、c = 74.63和3.2的衍射。晶体表现出近乎完美的半面形的双晶。不对称的晶体单元对应一个溶剂含量46.2%。水晶(TtKDGK-2)属于空间群P6 (3),晶胞参数a = b = 84.83, c =168.49, 2.25和期刊。展览部分半面的孪生,双分数的24.4%。晶体的TtKDGK绑定ATP (TtKDGK-ATP)= b = 84.72, c = 321.61和绑定KDG +ATP模拟AMP-PNP (TtKDGK-ATP-KDG)晶胞参数a = b = 84.32, c = 168.7,也准备和特征。

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