Crystallization and preliminary X-ray diffraction analysis of a thermostable endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3.

Yamaguchi A; Tada T; Nakaniwa TKitatani TTakao MSakai TNishimura K

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of a thermostable endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3.

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Crystallization and preliminary X-ray diffraction analysis of a thermostable endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3.

机译：结晶和初步的x射线衍射分析耐热性的endo-1 5-alpha-L-arabinanase从芽孢杆菌thermodenitrificans TS-3。

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A thermostable endo-1,5-alpha-L-arabinanase ABN-TS from Bacillus thermodenitrificans TS-3 with a molecular weight of 35 kDa was crystallized by the hanging-drop vapour-diffusion method using sodium citrate as a precipitant. The crystals were loop-mounted in a cryoprotectant solution containing 28%(w/v) sucrose and 1 M sodium citrate pH 6.0 and flash-cooled. Sucrose was selected as the most suitable cryoprotectant. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 40.3, b = 77.8, c = 89.7 A. The calculated V(M) based on one molecule per asymmetric unit was 2.0 A(3) Da(-1). A complete data set from a frozen crystal was collected to 1.9 A resolution using synchrotron radiation at SPring-8. A molecular-replacement solution was obtained using the structure of alpha-arabinanase 43A from Cellvibrio japonicus.

机译：一种耐热性的endo-1, 5-alpha-L-arabinanase ABN-TS从芽孢杆菌thermodenitrificans TS-3用分子量35 kDa的结晶的悬滴vapour-diffusion方法使用柠檬酸钠作为沉淀剂。在冷冻保护剂loop-mounted解决方案吗含28% (w / v)蔗糖和1米钠柠檬酸和flash-cooled pH值6.0。选择最合适的冷冻保护剂。水晶属于斜方晶系的空间群P2(1) 2(1) 2(1),晶胞参数=40.3, b = 77.8, c = 89.7。基于每一个分子不对称单位为2.0(3) Da(1)。水晶收集分辨率1.9使用在SPring-8同步辐射。molecular-replacement的解决方案是使用获得的alpha-arabinanase 43的结构Cellvibrio japonicus .

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第6期|1149-1151|共3页
作者
Yamaguchi A; Tada T; Nakaniwa TKitatani TTakao MSakai TNishimura K;
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Research Institute for Advanced Science and Technology, Osaka Prefecture University, Sakai, Osaka 599-8570, Japan.;

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正文语种英语
中图分类化学;
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sodium citrate; Cellvibrio japonicus; Geobacillus thermodenitrificansX-ray diffraction analysisX-raysCryoprotective AgentsCrystallization;

机译：thermodenitrificansX-ray衍射analysisX-raysCryoprotectiveAgentsCrystallization;

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Crystallization and preliminary X-ray diffraction analysis of a thermostable endo-1,5-alpha-L-arabinanase from Bacillus thermodenitrificans TS-3.

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