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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification and crystallization of two peroxisomal acyl-CoA oxidases from Arabidopsis thaliana.
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Expression, purification and crystallization of two peroxisomal acyl-CoA oxidases from Arabidopsis thaliana.

机译：的表达、纯化和结晶两个酶酰coa氧化酶类拟南芥。

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摘要

Two members of the acyl-CoA oxidase family from Arabidopsis thaliana have been cloned, overexpressed, purified and crystallized. Long-chain-specific acyl-CoA oxidase 1 crystals are characterized by a large variation in diffraction quality and non-isomorphous unit-cell parameters. The best crystals diffract to 2.0 A using synchrotron radiation, have unit-cell parameters a = 85.2, b = 117.0, c = 131.0 A, alpha = beta = gamma = 90 degrees and show P2(1)2(1)2(1) symmetry. There are two polypeptide chains in the asymmetric unit. Short-chain-specific acyl-CoA oxidase 4 crystals are trigonal, space group P3(1)21/P3(2)21, with unit-cell parameters a = b = 198.7, c = 149.6 A. The crystals are most likely to contain six polypeptide chains in the asymmetric unit. Freshly prepared acyl-CoA oxidase 4 crystals diffract to 3.9 A at cryogenic temperature at beamline I711, Max-Lab, but the diffraction quality degenerates after storage for only a few days in the crystallization drop. A selenomethionine-substituted form of the protein was produced and two-wavelength MAD data were collected at beamline BW7A, EMBL Outstation, Hamburg.
机译：两个酰基辅酶氧化酶的家人拟南芥被克隆,过表达,纯化,结晶。Long-chain-specific酰coa氧化酶1晶体的特点是一个大的变化衍射质量和non-isomorphous晶胞参数。使用同步辐射,晶胞参数= 85.2,= 117.0 b, c = 131.0,α=β=γ= 90度并显示P2(1) 2(1) 2(1)对称。链的不对称单元。Short-chain-specific酰coa氧化酶4晶体三方晶系的,空间群P3 (1) 21 / P3(2) 21日晶胞参数a = b = 198.7, c = 149.6。水晶是最有可能包含六个多肽链的不对称单元。刚做好的酰coa氧化酶4晶体衍射在低温温度3.9Max-Lab beamline I711,但衍射为只有少数质量退化后存储天的结晶。selenomethionine-substituted形式的蛋白质生产和two-wavelength疯了数据收集在beamline BW7A EMBL的分站,汉堡。

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