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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Twinned or not twinned, that is the question: crystallization and preliminary crystallographic analysis of the (2)F1(3)F1 module pair of human fibronectin.
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Twinned or not twinned, that is the question: crystallization and preliminary crystallographic analysis of the (2)F1(3)F1 module pair of human fibronectin.

机译：成双成对的成双成对的,这是一个问题:结晶和初步的晶体F1的分析(2)(3)F1模块对人类纤连蛋白。

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摘要

Human fibronectin (Fn) is a large multidomain protein found in the extracellular matrix and plasma. It is involved in many cellular processes, including cell adhesion and migration during embryogenesis and wound healing. The ability to bind Fn is a characteristic that has been demonstrated for a number of pathogens. For Staphylococcus aureus and Streptococcus pyogenes in particular, Fn-binding bacterial proteins (FnBPs) have been shown to mediate not only bacterial adhesion to host cells but also the uptake of bacteria by the cells. FnBPs interact with the amino-terminal region of Fn, where five type I ((1-5)F1) Fn modules are located. Although the structures of two F1 module pairs have been determined by NMR, no X-ray structures have been reported. To explore the conformational interactions between modules and the binding properties of FnBPs, the (2)F1(3)F1 module pair was crystallized using the vapour-diffusion method at 298 K. 12 X-ray diffraction data sets have been collected: six on an in-house rotating anode (three native, one Pt derivative and two peptide-bound) and six at synchrotron-radiation sources (two native and four derivative). Following analysis of these data, some of which have very high multiplicity (up to 50), probable space-group assignments were made (P42(1)2, P4(1)2(1)2 or P4(3)2(1)2) and the possibly twinned nature of the crystals was investigated using six different tests. The results presented here suggest that the crystals are not twinned.
机译：人类纤连蛋白(Fn)是一个大型的多畴的蛋白质在细胞外基质和等离子体。过程,包括细胞粘附和迁移在胚胎发生和伤口愈合。Fn是一个特点,结合的能力被证实的病原体。金黄色葡萄球菌和酿脓链球菌特别是,Fn-binding细菌蛋白(FnBPs)已被证明调解不仅细菌粘附宿主细胞也细菌的细胞的吸收。伴的Fn地区,五个I型((1 - 5)F1) Fn模块。两对F1模块的结构由核磁共振,没有x射线结构报道。模块之间的交互和绑定F1 FnBPs属性,(2)(3)对F1模块是使用vapour-diffusion结晶方法在298 k 12 x射线衍射数据集已经收集了:六内部旋转阳极(三个人,一个Pt导数和两个peptide-bound)和六个电脑同步来源(两个本地和四阶导数)。分析这些数据之后,其中的一些有很高的多样性(50),可能吗空间群作业了(第42页(1)2、P4(1) 2(1) 2或P4(3) 2(1) 2)和可能成双成对的晶体的性质研究使用六种不同的测试。这表明,晶体没有成双成对的。

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