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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate.
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Metal sites in 3,4-dihydroxy-2-butanone 4-phosphate synthase from Methanococcus jannaschii in complex with the substrate ribulose 5-phosphate.

机译：4-dihydroxy-2-butanone金属网站3日从产甲烷球菌属4-phosphate合酶jannaschii在复杂基质核酮糖5-phosphate。

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摘要

The crystal structure of Methanococcus jannaschii 3,4-dihydroxy-2-butanone 4-phosphate synthase in complex with the substrate ribulose 5-phosphate at a dimetal centre has recently been determined at 1.7 A resolution. The enzyme converts ribulose 5-phosphate into 3,4-dihydroxy-2-butanone 4-phosphate, while its C4 atom is released as formate. The resulting four-carbon body supplies all eight C atoms for the xylene moiety of riboflavin. Three of the four hydroxyl groups of ribulose 5-phosphate were coordinated by the metal ions. Based on crystallographic refinement, the metals were assigned as zinc and calcium, which were present in the crystallization buffer. Neither metal supports the enzymatic reaction. In the present study, the correctness of this assignment is assessed using anomalous diffraction data collected at the high-energy side of the zinc absorption edge (lambda = 1.2823 A). Only the three tentative zinc ions give strong peaks in an anomalous difference Fourier map (>20sigma), whereas the four tentative calcium ions do not show anomalous signals above the noise level. These results confirm the initial assignment. In addition, the resolution was improved to 1.55 A.
机译：晶体结构的产甲烷球菌属jannaschii3, 4-dihydroxy-2-butanone 4-phosphate合成酶与底物二磷酸核5-phosphate复杂在dimetal中心最近被确定1.7一项决议。4-dihydroxy-2-butanone 5-phosphate到3日4-phosphate,而C4原子被释放甲酸。所有八个C原子的二甲苯一半核黄素。核酮糖5-phosphate是协调的金属离子。金属被指定为锌和钙,在场的结晶缓冲区。无论是金属支持酶反应。目前的研究中,它的正确性的任务是评估使用异常在高能衍射数据收集锌的吸收边缘(λ= 1.2823)。只有三个试探性的锌离子傅里叶强峰在一个异常差异地图(> 20σ),而四个试探性的钙离子不显示异常信号噪音水平。初始赋值。提高到1.55。

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