Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex.

Bayrer J; Wan Z; Li BWeiss MA

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Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex.

机译：表达、结晶和初步的x射线果蝇转录的表征Doublesex因素。

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Doublesex (DSX) is a transcription factor in Drosophila melanogaster that regulates somatic sexual differentiation. Homologues have been found in diverse metazoans, suggesting a conserved role in development. Here, the expression, purification and crystallization of a novel 63-amino-acid dimerization domain and selenomethionine analogue are described. Native crystals belong to space group P2(1)2(1)2(1) and diffract to a resolution of 1.6 A with synchrotron radiation. Selenomethionine-containing crystals provide anomalous dispersion for phasing.

机译：Doublesex (DSX)是一个转录因子黑腹果蝇,调节躯体性分化。多样化的后生动物中发现,暗示保存在发展中的作用。的表达、纯化和结晶63 -氨基酸二聚作用域和小说硒代蛋氨酸模拟。水晶属于空间群P2 (1) 2 (1) 2 (1)衍射分辨率为1.6同步加速器辐射。Selenomethionine-containing晶体提供定相的反常色散。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第7期|1328-1330|共3页
作者
Bayrer J; Wan Z; Li BWeiss MA;
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作者单位

Case Western Reserve University, Cleveland, Ohio, USA.;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
Transcription Factors; anomalous dispersion; Selenomethionine, (S)-IsomerNOT OTHERWISE SPECIFIEDX-raysCrystallization;

机译：转录因子;异常dispersion;Selenomethionine, (S) -IsomerNOT否则SPECIFIEDX-raysCrystallization;

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Expression, crystallization and preliminary X-ray characterization of the Drosophila transcription factor Doublesex.

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