Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.

Li R; Ren H; Liang YDing HQiu SLu SAn JLi LLuo MZheng XSu XD

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.

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Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.

机译：蛋白质制备、结晶和初步的x射线分析人类的肾上腺蛋白质ad - 004。

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The adrenal gland protein AD-004 was identified in the human adrenal gland. Full-length AD-004 contains 172 amino acids, with a predicted molecular weight of about 20 kDa. In attempts to crystallize human AD-004, the gene was subcloned into a modified pET vector, pET21-DEST, with an N-terminal His(5) tag using the Gateway cloning system, followed by protein expression in Escherichia coli strain BL21(DE3). The protein was purified in two steps to near-homogeneity and was crystallized. The crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 99.56, c = 57.19 A. A complete 2.0 A data set has been collected at a rotating-anode X-ray source and structure determination is under way.

机译：肾上腺蛋白质广告- 004被确认人类的肾上腺。含有172个氨基酸,预测分子量约为20 kDa。结晶人类广告- 004,subcloned基因成一个修改宠物向量,pET21-DEST,氨基端(5)标签使用网关克隆系统,其次是蛋白质表达大肠杆菌菌株BL21 (DE3)。在两步纯化近乎均匀和是结晶。集团P6(1)或P6(5),晶胞参数A = b = 99.56, c = 57.19。一直在收集旋转阳极x射线源和结构测定。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第7期|1292-1294|共3页
作者
Li R; Ren H; Liang YDing HQiu SLu SAn JLi LLuo MZheng XSu XD;
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作者单位

National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, People's Republic of China.;

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正文语种英语
中图分类化学;
关键词
Human; X-ray analysis; Molecular weightscrystalsX-raysAdrenal Glands;

机译：人类、x射线分析、分子weightscrystalsX-raysAdrenal腺体;

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Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004.

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