Expression, purification, crystallization and preliminary X-ray analysis of alpha-L-arabinofuranosidase B from Aspergillus kawachii.

Miyanaga A; Koseki T; Matsuzawa HWakagi TShoun HFushinobu S

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Expression, purification, crystallization and preliminary X-ray analysis of alpha-L-arabinofuranosidase B from Aspergillus kawachii.

【24h】

Expression, purification, crystallization and preliminary X-ray analysis of alpha-L-arabinofuranosidase B from Aspergillus kawachii.

机译：表达,纯化,结晶初步的x射线分析alpha-L-arabinofuranosidase B曲霉属真菌

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

alpha-L-Arabinofuranosidase (EC 3.2.1.55) is one of the hemicellulases that cleave the glycosidic bonds between L-arabinofuranoside side chains and various oligosaccharides. In this study, the first crystallization and preliminary X-ray analysis of alpha-L-arabinofuranosidase B from Aspergillus kawachii IFO4308 (AkAbfB), a family 54 glycoside hydrolase, is described. Recombinant AkAbfB was expressed in Escherichia coli and Pichia pastoris. The native crystals of recombinant AkAbfB produced by P. pastoris belong to the orthorhombic space group P2(1)2(1)2(1) (unit-cell parameters a = 39.5, b = 98.2, c = 144.0 A) and diffracted X-rays to a resolution of 1.82 A.

机译：alpha-L-Arabinofuranosidase (EC 3.2.1.55)就是其中之一半纤维素酶的叫糖苷债券之间L-arabinofuranoside侧链各种低聚糖。首先结晶和初步的x射线分析alpha-L-arabinofuranosidase B54个糖苷水解酶,是描述。AkAbfB在大肠杆菌和表达毕赤酵母属pastoris。重组AkAbfB由p pastoris归属感斜方晶系的空间群P2 (1) 2 (1) 2 (1)(晶胞参数= 39.5,b = 98.2, c =144.0)和x射线衍射的决议1.82。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2004年第7期|1286-1288|共3页
作者
Miyanaga A; Koseki T; Matsuzawa HWakagi TShoun HFushinobu S;
展开▼
作者单位

Department of Biotechnology, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
Aspergillus; X-ray analysis; hemicellulaseAspergillus kawachiiX-raysCrystallization;

机译：分析;hemicellulaseAspergilluskawachiiX-raysCrystallization;

相似文献

外文文献
中文文献

1. Science Letters:Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii [J] . GAO Yong-gui, YAO Min, TANAKA Isao 浙江大学学报（英文版）（B辑：生物医学和生物技术） . 2005,第006期
2. A formal framework (Expression + Analysis) for network security [C] . Laborde R., Barrere F., Benzekri A. 2005 IEEE 61st Vehicular Technology Conference, 2005. VTC 2005-Spring . 2005

机译：网络安全的正式框架（Expression + Analysis）
3. Crystallization and preliminary X-ray diffraction analysis of phospholipase A(1) isolated from hornet (Vespa basalis) venom [O] . Chou, C.C., Hou, M.H., 侯明宏 2014

机译：Crystallization and preliminary X-ray diffraction analysis of phospholipase a(1) isolated from hornet (Vespa basalis) venom

Expression, purification, crystallization and preliminary X-ray analysis of alpha-L-arabinofuranosidase B from Aspergillus kawachii.

摘要

著录项

相似文献

相关主题

期刊订阅