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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Structure of macrophomate synthase.
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Structure of macrophomate synthase.

机译：macrophomate合成酶的结构。

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摘要

Macrophomate synthase (MPS) is an enzyme that catalyzes an extraordinarily complex conversion reaction, including two decarboxylations, two carbon-carbon bond formations and a dehydration, to form the benzoate analogue macrophomate from a 2-pyrone derivative and oxalacetate. Of these reactions, the two carbon-carbon bond formations are especially noteworthy because previous experiments have indicated that they proceed via a Diels-Alder reaction, one of the most widely used reactions in organic synthesis. The structural evidence that MPS catalyzes an intermolecular Diels-Alder reaction has been reported recently [Ose et al. (2003), Nature (London), 422, 185-189]. Interestingly, the tertiary structure as well as the quaternary structure of MPS are similar to those of 2-dehydro-3-deoxygalactarate (DDG) aldolase, a carbon-carbon bond-forming enzyme that catalyzes the reversible reaction of aldol condensation/cleavage. Here, the structure of MPS is described in detail and compared with that of DDG aldolase.Both enzymes have a (beta/alpha)(8)-barrel fold and are classified as belonging to the enolase superfamily based on their reaction strategy. The basic principles for carbon-carbon bond formation used by both MPS and DDG aldolase are the same with regard to trapping the enolate substrate and inducing subsequent reaction. The major differences in the active sites between these two enzymes are the recognition mechanisms of the second substrates, 2-pyrone and DDG, respectively.
机译：Macrophomate合成酶(MPS)是一种酶,这种酶催化一个极其复杂的转换反应,包括两个脱酸,两个碳碳键的形成和脱水,形成了苯甲酸模拟macrophomate从2-pyrone导数和oxalacetate。两个碳碳键的形成反应,尤其值得注意,因为之前通过实验表明,他们继续进行Diels-Alder反应,最广泛之一使用反应在有机合成。结构性议员催化一个的证据分子间Diels-Alder反应近日报道(大阪证交所et al .(2003),自然(伦敦),422,185 - 189]。三级结构以及第四纪议员的结构是相似的2-dehydro-3-deoxygalactarate醛缩酶(DDG)碳碳bond-forming酶催化醇醛的可逆反应冷凝/乳沟。详细描述和比较的吗DDG醛缩酶。(β/α)(8)桶褶皱和被归类为属于烯醇酶总科的基础上他们的反应策略。国会议员和所使用的碳碳键的形成DDG醛缩酶对于捕获相同烯醇化物衬底和诱导后续的反应。网站这两个酶之间第二基板的识别机制,分别2-pyrone和干玉米酒糟。

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