Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays.

Kopecny D; Briozzo P; Joly NHouba Herin NMadzak CPethe CLaloue M

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays.

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Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays.

机译：净化、结晶和初步的x射线衍射研究重组细胞分裂素氧化酶玉蜀黍。

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Cytokinins are hormones that are involved in plant growth and development. They are irreversibly degraded by cytokinin oxidases/dehydrogenases, flavoenzymes which contain a covalently bound flavine adenine dinucleotide (FAD) cofactor. Cytokinin oxidase from Zea mays (ZmCKO1) was overexpressed in the yeast Yarrowia lipolytica, purified (molecular weight 69 kDa) and crystallized using the hanging-drop method. Crystals belong to the monoclinic space group C2, with unit-cell parameters a = 250.6, b = 50.6, c = 51.5 A, beta = 94.1 degrees. A complete data set has been collected at 100 K to 1.95 A resolution on an X-ray synchrotron source.

机译：细胞分裂素是参与植物的荷尔蒙增长和发展。退化的细胞分裂素氧化酶类/脱氢酶,黄素酶含有一个共价结合黄素腺嘌呤二核苷酸(时尚)代数余子式。细胞分裂素氧化酶从玉蜀黍(ZmCKO1)在酵母Yarrowia lipolytica,净化(分子量69 kDa)使用悬滴法结晶。属于单斜晶体空间群C2,晶胞参数= 250.6,= 50.6 b, c= 51.5,β= 94.1度。1.95设置已经收集了在100 K关于x射线同步源的决议。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第8期|1500-1501|共2页
作者
Kopecny D; Briozzo P; Joly NHouba Herin NMadzak CPethe CLaloue M;
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Laboratoire de Biologie Cellulaire, INRA, Route de Saint-Cyr, 78026 Versailles CEDEX, France.;

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正文语种英语
中图分类化学;
关键词
Yarrowia lipolytica; Cytokinins; Purificationcytokinin oxidaseX-rays;

机译：oxidaseX-rays;

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Purification, crystallization and preliminary X-ray diffraction study of a recombinant cytokinin oxidase from Zea mays.

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