Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic archaeal sulredoxin having the unique Rieske (2Fe-2S) cluster environment.

Uchiyama T; Kounosu A; Sato TTanaka NIwasaki TKumasaka T

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic archaeal sulredoxin having the unique Rieske (2Fe-2S) cluster environment.

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Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic archaeal sulredoxin having the unique Rieske (2Fe-2S) cluster environment.

机译：结晶和初步的x射线衍射hyperthermophilic的研究热点sulredoxin拥有独特的Rieske (2 fe-2s)集群环境。

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The hyperthermophilic archaeal sulredoxin from Sulfolobus tokodaii is a water-soluble high-potential Rieske [2Fe-2S] protein with unique pH-dependent redox properties compared with its mesophilic homologues in cytochrome bc(1)/b(6)f complexes. The oxidized recombinant sulredoxin has been crystallized by the hanging-drop vapour-diffusion method using 30%(v/v) polyethylene glycol 400, 0.1 M cadmium chloride and 0.1 M sodium acetate pH 4.6. The crystals diffracted to beyond 2.0 A resolution and belong to the cubic space group F4(1)32, with unit-cell parameter a = 163.00 +/- 0.05 A. The asymmetric unit contains one sulredoxin molecule. Three-wavelength MAD data were collected.

机译：的hyperthermophilic热点sulredoxin硫化叶菌tokodaii水溶性高潜力的Rieske [2 fe-2s]蛋白质独特的pH-dependent氧化还原性能比较嗜中温同系物在细胞色素公元前(1)f / b(6)复合物。sulredoxin结晶了悬滴vapour-diffusion方法使用30% (v / v)聚乙二醇400、0.1镉氯乙酸钠和0.1米的pH值4.6。晶体衍射超出2.0决议和属于立方空间群F4(1) 32岁晶胞参数= 163.00 + / - 0.05。不对称单元包含一个sulredoxin分子。Three-wavelength疯狂的数据收集。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第8期|1487-1489|共3页
作者
Uchiyama T; Kounosu A; Sato TTanaka NIwasaki TKumasaka T;
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Department of Life Science, Tokyo Institute of Technology, Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.;

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正文语种英语
中图分类化学;
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PH; Redox properties; EnvironmentsX-ray diffractionSulfolobus tokodaiiX-raysCrystallization;

机译：PH值;氧化还原性质;EnvironmentsX-raydiffractionSulfolobustokodaiiX-raysCrystallization;

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Crystallization and preliminary X-ray diffraction studies of the hyperthermophilic archaeal sulredoxin having the unique Rieske (2Fe-2S) cluster environment.

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