Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa.

Cobessi D; Celia H; Folschweiller NHeymann MSchalk IAbdallah MPattus F

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Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa.

机译：结晶和初步的x射线分析外膜pyoverdine受体FpvA铜绿假单胞菌。

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FpvA, the pyoverdine outer-membrane receptor from Pseudomonas aeruginosa, is involved in iron uptake when bacteria grow under iron limitation. Crystals of the in vivo pyoverdine-loaded FpvA were obtained under several crystallization conditions using different detergents. A native data set was collected at 3.6 A resolution and a three-wavelength MAD data set was collected at 3.6 A resolution using crystals of selenomethionine-substituted protein. The crystals grew under similar conditions and both belong to space group C2, but have different unit-cell parameters.

机译：FpvA, pyoverdine外膜受体铜绿假单胞菌、参与铁在铁限制的条件下吸收当细菌生长。晶体的体内pyoverdine-loaded FpvA得到以下几个结晶条件下使用不同的洗涤剂。数据集在3.6决议和收集three-wavelength疯狂数据集收集3.6使用晶体的一项决议selenomethionine-substituted蛋白质。晶体生长在类似的条件下,两种属于空间群C2,但有不同的晶胞参数。

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《Acta crystallographica.Section D. Biological crystallography》 |2004年第8期|1467-1469|共3页
作者
Cobessi D; Celia H; Folschweiller NHeymann MSchalk IAbdallah MPattus F;
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作者单位

Departement Recepteurs et Proteines Membranaires, UPR 9050 CNRS, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard Sebastien Brant, 67410 Illkirch, France.;

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中图分类化学;
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Ferric pyoverdin; X-ray analysis; Pseudomonas aeruginosacrystalsCrystallizationReceptorDataset;

机译：铁pyoverdin;x射线分析,假单胞菌aeruginosacrystalsCrystallizationReceptorDataset;

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Crystallization and preliminary X-ray analysis of the outer membrane pyoverdine receptor FpvA from Pseudomonas aeruginosa.

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