Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization.

Adachi H; Takano K; Morikawa MKanaya SYoshimura MMori YSasaki T

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization.

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Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization.

机译：应用程序的两个液体系统sitting-dropvapour-diffusion蛋白质结晶。

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A new method of protein crystallization, the floating-drop vapour-diffusion technique, has been developed. The method combines the traditional sitting-drop vapour-diffusion technique and a novel two-liquid system. A crystallization drop composed of a mixture of sample and reagent floats on an insoluble and very dense liquid. Protein crystals are grown by vapour diffusion at the interface of the two liquids. The method makes it possible to remove the crystals easily without causing mechanical damage. This approach also significantly reduces the time and cost compared with the hanging-drop technique, which is presently the most popular method for protein crystallization.

机译：一个新的蛋白质结晶的方法,floating-drop vapour-diffusion技术被开发出来。传统sitting-drop vapour-diffusion技术和小说两个液体系统。结晶的混合物组成的下降样品和试剂漂浮在一个不溶性非常密集的液体。蒸汽扩散界面的两个液体。晶体容易不会引起机械损害。时间和成本而悬滴技术,是目前最受欢迎的蛋白质结晶的方法。

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来源
《Acta crystallographica.Section D. Biological crystallography》 |2003年第1期|194-196|共3页
作者
Adachi H; Takano K; Morikawa MKanaya SYoshimura MMori YSasaki T;
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作者单位

Department of Electrical Engineering, Graduate School of Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
HEAVY LIQUID; Protein; diffusion techniqueCrystallization;

机译：重液;蛋白质;扩散techniqueCrystallization;

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Application of a two-liquid system to sitting-drop vapour-diffusion protein crystallization.

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