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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystals of trp repressor suitable for high-resolution neutron Laue diffraction studies.
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Crystals of trp repressor suitable for high-resolution neutron Laue diffraction studies.

机译：晶体的阻遏适合高分辨率的中子衍射研究劳厄。

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摘要

Crystallization and preliminary neutron-diffraction measurements of wild-type variant Val58-->Ile of the Escherichia coli trp repressor are reported. A vapor-diffusion chamber suitable for initial protein-solution Volumes in the range 0.2-0.5 ml was used to grow cube-shaped crystals with edge dimensions in the range 0.8-1.4 mm. Neutron Laue measurements to a nominal resolution of 2.1 A were recorded from a D(2)O-exchanged crystal using the LADI instrument at ILL. These results demonstrate that it will be possible for the first time to obtain a full-atom neutron structural model of a DNA-binding protein plus its associated solvent. Direct observation of hydrogen bonding between protein and solvent should enhance understanding of the role of solvent in protein-DNA recognition.
机译：结晶和初步中子衍射测量野生型变体Val58——>大肠杆菌trp Ile抑制因子报告。适合最初的蛋白质溶剂量范围0.2 - -0.5 ml被用来成长方形晶体的边缘尺寸范围0.8 - -1.4毫米。名义上的分辨率2.1的记录D(2)使用LADI O-exchanged水晶乐器在生病。可能第一次获得full-atom中子结构模型的dna结合蛋白质加上相关的溶剂。蛋白质和溶剂之间的氢键应加强理解的作用溶剂在protein-DNA识别。

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