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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Phasing the AP2 core complex with Xe, Hg and Se.
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Phasing the AP2 core complex with Xe, Hg and Se.

机译：逐步Xe的AP2核心复杂,汞和硒。

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摘要

The 200 kDa core of the heterotetrameric AP2 clathrin adaptor complex was phased with xenon, mercury and selenomethionine derivatives. The phasing has been analysed in retrospect to determine how many of the derivative data sets were necessary for optimum phasing and what features were most useful in deciding which derivatives would make a positive contribution. The relative contributions of the different derivatives indicate that the most important phasing came from the two Xe data sets collected at long wavelengths (1.74 and 1.98 A) to enhance the anomalous signal. The mercury derivatives were less powerful but made a useful contribution, although inclusion of a poor second wavelength set was detrimental, probably because of radiation damage. The SeMet data were less useful for phasing because of incomplete incorporation of selenium owing to the expression conditions needed, but they were useful in chain-tracing.
机译：200 kDa的核心heterotetrameric AP2网格蛋白适配器复杂与氙气分阶段,汞和硒代蛋氨酸衍生品。逐步分析了回想起来确定有多少的导数数据集有必要逐步优化和什么在决定哪些最有用的特性衍生品将作出积极的贡献。的相对贡献不同衍生品表明最重要定相来自两个Xe数据集收集长波长(1.74和1.98)来提高异常信号。不太强大,但做一个有用的贡献,尽管包含第二波长是有害的,可能是因为辐射的危害。定相的有用,因为不完整将硒由于表达式条件需要,但他们是有用的chain-tracing。

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