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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >A new class of lanthanide complexes to obtain high-phasing-power heavy-atom derivatives for macromolecular crystallography.
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A new class of lanthanide complexes to obtain high-phasing-power heavy-atom derivatives for macromolecular crystallography.

机译：一个新类的镧系配合物来获得high-phasing-power重原子衍生物对大分子晶体学。

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摘要

Because of their intense white lines and large f" values, lanthanide atoms are of great interest for solving structures of biological macromolecules using single-wavelength anomalous diffraction (SAD) or multiple-wavelength anomalous diffraction (MAD) methods. In this work, a series of seven gadolinium complexes are described which provide excellent derivatives for anomalous diffraction experiments in biological systems. These highly soluble lanthanide complexes can easily be introduced into protein crystals either by soaking or by co-crystallization, without significantly affecting the crystallization conditions, by employing highly concentrated complex solutions ( approximately 100 mM). De novo phasing by the SAD method was carried out with several proteins of known as well as previously unknown structures by employing this new class of heavy-atom compounds. Diffraction data were collected either with a laboratory source, making use of the high anomalous signal (f" = 12 e(-)) of gadolinium with Cu Kalpha radiation, or with synchrotron radiation at the peak of the gadolinium L(III) absorption edge, which exhibits a strong white line (lambda = 1.711 A, f" = 28 e(-)). Using one of these gadolinium complexes, Gd-HPDO3A, the structure of a bacterial chimeric ornithine carbamoyl transferase, OTCase3630, a dodecameric protein of 450 kDa, was determined. Employed with the SAD method, these seven complexes could be of particular interest for high-throughput macromolecular crystallography.
机译：因为他们强烈的白线和大f”值,镧系元素原子的极大的兴趣求解结构的生物大分子用单波长反常衍射(SAD)或多波长反常衍射(疯狂)方法。工作,一系列七钆配合物描述提供优秀的衍生品反常衍射实验生物系统。配合物可以很容易地引入蛋白质晶体通过浸泡或co-crystallization,没有明显影响结晶条件,采用高度集中复杂的解决方案(大约100毫米)。与几个蛋白质的方法进行已知和未知的结构使用这个新类的重原子的化合物。衍射数据收集的实验室资源,利用高异常信号(f = 12 e(-))的钆与铜Kalpha辐射,或同步辐射的峰值钆L (3)吸收边,表现出强烈的白色线(λ= 1.711,f = 28 e(-))。Gd-HPDO3A,这些钆配合物细菌嵌合鸟氨酸的结构[氨基甲酰转移酶、OTCase3630 dodecameric蛋白质450 kDa的决心。悲伤的方法,这七个复合物可以的特别感兴趣的高通量大分子晶体学。

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