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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Oils used in microbatch crystallization do not remove a detergent from the drops they cover.
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Oils used in microbatch crystallization do not remove a detergent from the drops they cover.

机译：油用于microbatch结晶删除一个洗涤剂滴它们覆盖。

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摘要

In microbatch crystallizations, a small volume of protein solution is placed in contact with a large volume of oil. Detergents present in the water phase may be expected to migrate into the oil phase, which could have effects on the protein in solution. A new method is described in which detergent partitioning into the oil can be checked. The accuracy of this method is sufficiently high to estimate even very low partitioning coefficients. The measurements indicate that in the case of dodecyl maltoside, no significant loss of detergent will occur.
机译：在microbatch结晶方法,体积小蛋白质溶液接触大量的石油。可能会迁移到水阶段油相,这可能有影响蛋白质在溶液中。洗涤剂分区到石油可以吗检查。足够高估计甚至很低分区系数。表明,在十二烷基maltoside的情况下,没有清洁剂将发生的重大损失。

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