首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization of (Fe(3)S(4))-ferredoxin from the hyperthermophile archaeon Pyrococcus furiosus.
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Crystallization of (Fe(3)S(4))-ferredoxin from the hyperthermophile archaeon Pyrococcus furiosus.

机译:结晶(Fe(3)(4))的铁氧还蛋白

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摘要

Recombinant Pyrococcus furiosus ferredoxin with a [Fe(3)S(4)]-cluster was crystallized through steps of optimization and X-ray diffraction data were collected from several crystal forms. Flat plate-like crystals were grown by hanging-drop vapour diffusion. The precipitant used was 30% PEG 400; the pH was varied between 7.0 and 8.0 and hexaamminecobalt(III) chloride was essential for crystal formation. The best crystals belong to space group P2(1), with unit-cell parameters a = 31.23, b = 47.51, c = 52.03 A, beta = 103.86 degrees, and diffract to 1.5 A resolution.
机译:重组海床furiosus铁氧还蛋白的(Fe(3)(4))集群是结晶优化和x射线衍射数据的步骤收集来自几个晶体形式。增加了悬滴状晶体蒸汽扩散。挂钩400;氯化和hexaamminecobalt (III)是至关重要的对晶体的形成。空间群P2(1),一个晶胞参数= 31.23, b = 47.51, c = 52.03,β= 103.861.5度,衍射分辨率。

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