...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral.
【24h】

The production, purification and crystallization of a pocilloporin pigment from a reef-forming coral.

机译：生产、纯化和结晶从造礁的pocilloporin色素珊瑚。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Reef-building corals contain fluorescent pigments termed pocilloporins that function by regulating the light environment of coral and acting as a photoprotectant in excessive sunlight. These pocilloporins are related to the monomeric green fluorescent protein and the tetrameric DsRed fluorescent proteins, which have widespread use as biotechnological tools. An intensely blue-coloured pocilloporin, termed Rtms5, was expressed in Escherichia coli, purified and crystallized. Rtms5 was shown to be tetrameric, with deep blue crystals that diffract to 2.2 A resolution and belong to space group I4(1)22. The colour of this pocilloporin was observed to be sensitive to pH and a yellow (pH 3.5) and a red form (pH 4.5) of Rtms5 were also crystallized. These crystals belong to space group P4(2)22 and diffract to 2.4 A resolution or better.
机译：造礁珊瑚含有荧光颜料称为pocilloporins函数通过调节珊瑚和作为的光环境photoprotectant过度阳光。pocilloporins相关单体的绿色荧光蛋白和四聚物的安全域荧光蛋白,广泛使用随着生物技术工具。,称为Rtms5 blue-coloured pocilloporin大肠杆菌中表达,纯化和结晶。深蓝晶体衍射2.2分辨率和属于空间群预告(1)22。颜色pocilloporin被观察到敏感的pH值和一个黄色(pH值3.5)和一个红色的的形式(pH值4.5)Rtms5也结晶。这些晶体属于空间群P4 (2) 22衍射分辨率2.4或更好。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号