Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.

Cockburn JJ; Bamford JK; Grimes JMBamford DHStuart DI

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.

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Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.

机译：结晶的membrane-containing噬菌体在石英PRD1毛细血管蒸汽扩散。

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Crystals of bacteriophage PRD1, a virus containing an internal lipid bilayer, have been grown in thin-walled quartz capillary tubes by vapour diffusion as a means of eliminating mechanical handling of the crystals during data collection. It has been found that the addition of polyethylene glycol 20 000 (PEG 20K) to the mother liquor that bathes the crystals allows far higher resolution diffraction intensities to be observed. Growing and treating the crystals in this way has produced a small number of crystals which are particularly amenable to X-ray diffraction analysis.

机译：晶体的噬菌体PRD1,包含病毒一个内部脂质双分子层,一直在增长由蒸汽薄壁石英毛细管扩散的消除机械在数据采集处理的晶体。人们已经发现,添加聚乙二醇000 (PEG 20 k)母液,沐浴晶体允许高分辨率衍射强度观察到。这种方式产生了少量的晶体这是特别适合x射线衍射分析。

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《Acta crystallographica.Section D. Biological crystallography》 |2003年第3期|538-540|共3页
作者
Cockburn JJ; Bamford JK; Grimes JMBamford DHStuart DI;
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作者单位

The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Headington, Oxford OX3 7BN, England, and Oxford Centre for Molecular Sciences, Central Chemistry Building, South Parks Road, Oxford OX1 3QH, England.;

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正文语种英语
中图分类化学;
关键词
Enterobacteria phage PRD1; Quartz; crystal structurecrystalsVaporsmother liquorBacteriophagesX-ray diffraction analysisCrystallization;

机译：肠道菌噬菌体PRD1;石英;晶体structurecrystalsVaporsmotherliquorBacteriophagesX-ray衍射analysisCrystallization;

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Crystallization of the membrane-containing bacteriophage PRD1 in quartz capillaries by vapour diffusion.

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