Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli.

Chimento DP; Mohanty AK; Kadner RJWiener MC

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Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli.

机译：结晶和初始的x射线衍射BtuB,膜氰钴胺素转运体的积分大肠杆菌。

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BtuB, the cobalamin transporter from Escherichia coli, has been overexpressed, purified and crystallized. The purified protein was solubilized in n-octyl tetraoxyethylene (C(8)E(4)) and was crystallized using sitting-drop vapor diffusion with PEG 3350 and magnesium acetate as precipitants (pH 6.5). Two crystal forms have been obtained. Crystal type I belongs to space group P3(1)21, with unit-cell parameters a = b = 81.6, c = 210.0 A, alpha = beta = 90, gamma = 120 degrees. Crystal type II belongs to space group P3(1)21, with unit-cell parameters a = b = 81.6, c = 226.0 A, alpha = beta = 90, gamma = 120 degrees. Each crystal form contains a monomer in the asymmetric unit. Diffraction for crystal type I extends to 2.0 A and diffraction for crystal type II extends to 2.7 A. Both crystal forms are suitable for structure determination.

机译：从大肠BtuB,钴胺素转运体杆菌、过表达,纯化和结晶。随着在n-octyl tetraoxyethylene(C (8) E(4)),固定使用sitting-drop蒸汽扩散与PEG 3350和醋酸镁作为沉淀剂(pH值6.5)。晶体形式。属于空间群P3(1) 21日晶胞参数a = b = 81.6, c = 210.0,α=β= 90,γ= 120度。属于空间群P3(1) 21日晶胞参数a = b = 81.6, c = 226.0,α=β= 90,γ= 120度。不对称单元包含一个单体。衍射晶体类型我延伸到2.0和衍射晶体II型扩展2.7。结构的决心。

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《Acta crystallographica.Section D. Biological crystallography》 |2003年第3期|509-511|共3页
作者
Chimento DP; Mohanty AK; Kadner RJWiener MC;
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Department of Microbiology, University of Virginia, Charlottesville, Virginia 22908, USA.;

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正文语种英语
中图分类化学;
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Escherichia coli; Vitamin B 12; cell parametermagnesium acetatecrystal structurecrystalsCobalamincrystal outlineCrystallization;

机译：大肠杆菌,维生素B 12;细胞parametermagnesium acetatecrystaloutlineCrystallization;

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Crystallization and initial X-ray diffraction of BtuB, the integral membrane cobalamin transporter of Escherichia coli.

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