Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377.

Riboldi Tunnicliffe A; Bent CJ; Isaacs NWMitchell TJ

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377.

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Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377.

机译：净化、结晶和初步的粪肠球菌的x射线分析蛋白EF0377。

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The EF0377 gene of Enterococcus faecalis was cloned and overexpressed in Escherichia coli. The protein has been purified and crystallized in three forms. Type III crystals belong to space group P2(1), with unit-cell parameters a = 72.11, b = 94.97, c = 80.77 A, beta = 111.93 degrees. There are four molecules per asymmetric unit and diffraction is observed to beyond 1.65 A under cryoconditions (100 K) using synchrotron radiation. An almost complete set of X-ray diffraction data was collected to 1.9 A from the native crystal.

机译：粪肠球菌的EF0377基因克隆并在大肠杆菌中。蛋白质纯化和结晶三种形式。集团P2(1),晶胞参数= 72.11,b = 94.97, c = 80.77,β= 111.93度。每个不对称单位和有四个分子衍射观察到超出1.65使用同步加速器cryoconditions (100 K)辐射。1.9从衍射数据收集原生晶体。

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《Acta crystallographica.Section D. Biological crystallography》 |2003年第4期|755-757|共3页
作者
Riboldi Tunnicliffe A; Bent CJ; Isaacs NWMitchell TJ;
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正文语种英语
中图分类化学;
关键词
Enterococcus faecalis; X-ray analysis; Purificationsynchrotron radiationX-ray diffraction dataX-raysCrystallization;

机译：粪肠球菌;x射线分析;Purificationsynchrotron radiationX-ray衍射dataX-raysCrystallization;

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Purification, crystallization and preliminary X-ray analysis of the Enterococcus faecalis protein EF0377.

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