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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification, characterization and preliminary X-ray crystallographic studies on Neiserria gonorrhoeae Gly1ORF1.
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Purification, characterization and preliminary X-ray crystallographic studies on Neiserria gonorrhoeae Gly1ORF1.

机译：纯化、表征和初步的x射线晶体Neiserria研究

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摘要

Gly1ORF1 is a protein produced by the two pathogenic Neisseria species, N. gonorrhoeae and N. meningitidis, but not by commensal Neisseria, suggesting that it may be involved in pathogenesis. The protein has a signal sequence that is cleaved, is associated with outer membrane fractions of N. gonorrhoeae (GC) and is found in spent media and in outer-membrane fractions when expressed in Escherichia coli. GC strains with null mutations of the gly1 locus have increased toxicity to human fallopian tubes in organ culture, suggesting that Gly1ORF1 may alter the amount or properties of toxic moieties produced by GC [Arvidson et al. (1999), infect. Immun. 67, 643-652]. In an effort to understand the function of Gly1ORF1 and its role in pathogenesis, structural biology studies have been initiated. Here, the purification, characterization by dynamic light scattering, crystallization and preliminary X-ray crystallographic studies of recombinant Gly1ORF1 are reported. Dynamic light scattering indicated the protein to be a dimer in solution. The crystals belonged to space group P6(3), with unit-cell parameters a = 95.2, b = 95.2, c = 83.7 A and two molecules per asymmetric unit. The crystals diffracted to 2.4 A using a conventional X-ray source.
机译：Gly1ORF1是一种蛋白质产生的两个致病性奈瑟氏菌属物种:球菌脑膜炎奈瑟菌,但不是同桌的脑膜炎,这表明它可能参与发病机理。裂解,与外部相关联膜分数n .球菌(GC)和中发现了媒体和外膜分数在大肠杆菌中表达。与零gly1位点的突变菌株增加了毒性对人类输卵管吗在器官培养,这表明Gly1ORF1可能改变量或有毒根的属性由GC [Arvidson et al。(1999),感染。Immun。67年,643 - 652]。Gly1ORF1及其作用的功能发病机理、结构生物学的研究被启动。描述由动态光散射,结晶和初步的x射线重组Gly1ORF1晶体的研究报告。蛋白质在溶液中二聚体。晶体属于空间群P6 (3)晶胞参数= 95.2,= 95.2 b, c = 83.7一个和两个分子不对称单位。使用传统的晶体衍射,2.4x射线源。

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