Crystallization and X-ray diffraction data analysis of leukotriene A4 hydrolase from Saccharomyces cerevisiae.

Andersson B; Kull F; Haeggstrom JZThunnissen MM

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and X-ray diffraction data analysis of leukotriene A4 hydrolase from Saccharomyces cerevisiae.

【24h】

Crystallization and X-ray diffraction data analysis of leukotriene A4 hydrolase from Saccharomyces cerevisiae.

机译：结晶和x射线衍射数据分析白三烯A4水解酶酿酒酵母。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

The Saccharomyces cerevisiae leukotriene A4 (LTA4) hydrolase (scLTA4 hydrolase) has been crystallized in order to study the two activities of LTA4 hydrolase in an evolutionary perspective. Single well diffracting crystals are obtained after switching from the hanging-drop method to liquid-liquid diffusion in capillaries using PEG 8000 as precipitant. These crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 70.8, b = 98.1, c = 99.2 A. Intensity data to 2.3 A resolution were collected from a native scLTA4 hydrolase crystal using synchrotron radiation. A molecular-replacement solution was obtained using the human LTA4 hydrolase structure and the program BEAST.

机译：酿酒酵母的白三烯A4 (LTA4)水解酶(scLTA4水解酶)为了研究结晶两种活动LTA4水解酶的进化的观点。单井衍射晶体从悬滴法转向液-液扩散在毛细血管使用挂钩8000年作为沉淀剂。空间群P2(1) 2(1) 2(1),与单胞参数= 70.8,b = 98.1, c = 99.2。2.3强度数据收集的决议从本地scLTA4水解酶晶体同步加速器辐射。解决方案是使用人类LTA4获得水解酶的结构和程序的野兽。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2003年第6期|1093-1095|共3页
作者
Andersson B; Kull F; Haeggstrom JZThunnissen MM;
展开▼
作者单位

Department of Biochemistry and Biophysics, Stockholm University, S-106 91 Stockholm, Sweden.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
Saccharomyces cerevisiae; Leukotriene A4; X-ray diffraction analysisliquidsCrystallization;

机译：衍射analysisliquidsCrystallization;

相似文献

外文文献
中文文献

1. Science Letters:Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii [J] . GAO Yong-gui, YAO Min, TANAKA Isao 浙江大学学报（英文版）（B辑：生物医学和生物技术） . 2005,第006期
2. X-Ray Powder Diffraction Data—Identification and Structure Elucidation of Unknown Stone—Rietveld Refinement Approach [J] . Munusamy Baskar, Anju Patania 生物物理化学（英文） . 2020,第004期
3. Crystallization and preliminary X-ray diffraction analysis of phospholipase A(1) isolated from hornet (Vespa basalis) venom [O] . Chou, C.C., Hou, M.H., 侯明宏 2014

机译：Crystallization and preliminary X-ray diffraction analysis of phospholipase a(1) isolated from hornet (Vespa basalis) venom

Crystallization and X-ray diffraction data analysis of leukotriene A4 hydrolase from Saccharomyces cerevisiae.

摘要

著录项

相似文献

相关主题

期刊订阅