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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Using natural seeding material to generate nucleation in protein crystallization experiments.
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Using natural seeding material to generate nucleation in protein crystallization experiments.

机译：使用天然播种材料生成在蛋白质结晶成核实验。

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摘要

The nucleation event in protein crystallization is a part of the process that is poorly controlled. It is generally accepted that the protein should be in the metastable phase for crystal growth, but for nucleation higher levels of saturation are needed. Formation of nuclei in bulk solvent requires interaction of protein molecules until a critical size of aggregate is created. In many crystallization experiments sufficiently high levels of saturation are not reached to allow this critical nucleation event to occur. If an environment can be created that favours a higher local concentration of macromolecules, the energy barrier for nucleation may be lowered. When seeds are introduced at lower levels of saturation in a crystallization experiment, nucleation may be facilitated and crystal growth initiated. In this study, the use of natural materials as stable seeds for nucleation has been investigated. The method makes it possible to introduce seeds into crystallization trials at any stage of the experiment using both microbatch and vapour-diffusion methods.
机译：蛋白质结晶的成核事件控制不佳的过程的一部分。人们普遍认为蛋白质在晶体生长的亚稳相,但对于成核更高水平的饱和是必要的。需要相互作用的蛋白质分子,直到一个创建聚合的关键尺寸。结晶实验足够高饱和水平没有达到允许这个临界成核事件发生。环境可以创建支持更高当地的大分子浓度,能量障碍对成核可能会降低。介绍了在低水平的饱和吗结晶实验中,成核促进和晶体生长。研究中,使用天然材料稳定成核的种子已经调查。方法可以引入种子在任何阶段的结晶实验使用microbatch和实验vapour-diffusion方法。

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