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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of post-fusion six-helix bundle core structure from Newcastle disease virus F protein.
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Crystallization and preliminary X-ray diffraction analysis of post-fusion six-helix bundle core structure from Newcastle disease virus F protein.

机译：结晶和初步的x射线衍射分析post-fusion six-helix包的核心从新城鸡瘟病毒F蛋白结构。

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摘要

Fusion of virus members from the Paramyxoviridae family involves two glycoproteins. They are termed attachment glycoprotein (HN, H or G) and fusion protein (F). The F protein contains two highly conserved heptad-repeat (HR) regions, HR1 and HR2. Through conformational changes in the F protein, HR1 and HR2 are believed to form a stable six-helix coiled-coil bundle during the membrane-fusion process. However, no crystal structure has yet been documented for this state in the Newcastle disease virus (NDV, a member of the Paramyxoviridae family) F protein, despite the recent success on its F(0) crystal structure (Chen et al., 2001), which was thought to represent the pre-fusion conformation of F glycoprotein. In this study, a single-chain polypeptide constructed by linking two truncated HR regions of the NDV F protein has been expressed, purified and crystallized by means of the hanging- or sitting-drop vapour-diffusion method. Crystals in hexagonal and trapezoid forms with a resolution limit of 2.6 A wereobtained. These crystals belonged to space group C2, with unit-cell parameters a = 66.4, b = 38.2, c = 102.0 A, beta = 100.2 degrees. Crystals in the rhombic form with a resolution limit of 2.5 A were also obtained. These crystals belonged to space group P2(1), with unit-cell parameters a = 59.0, b = 31.9, c = 62.3 A, beta = 117.0 degrees. This will add to the repertoire of viral fusion protein post-fusion state structures and help further the understanding of the molecular mechanism of enveloped virus fusion.
机译：副黏液病毒科的成员的融合病毒家庭涉及两个糖蛋白。H和G称为附件糖蛋白(HN)融合蛋白(F), F蛋白包含两个高度保守的heptad-repeat(人力资源)地区,HR1上和HR2。蛋白质、HR1上和HR2被认为形成稳定six-helix卷曲螺旋包中膜融合过程。结构尚未记录的状态鸡新城疫病毒(NDV的一员副粘病毒科)F蛋白,尽管最近的成功在其F(0)的晶体结构(陈et al ., 2001),它被认为代表F的pre-fusion构象糖蛋白。多肽由连接两个截断人力资源地区的NDV F蛋白表达,纯化,结晶的悬挂或sitting-drop vapour-diffusion方法。wereobtained 2.6的分辨率极限。这些晶体属于空间群C2晶胞参数= 66.4,b = 38.2, c =102.0,β= 100.2度。菱形2.5分辨率的极限形式也获得了。空间群P2(1),晶胞参数=59.0, b = 31.9, c = 62.3,β= 117.0度。这将增加病毒融合的曲目蛋白质post-fusion状态结构和帮助进一步的理解分子包膜病毒融合机制。

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