New insight into the haemoglobin superfamily: preliminary crystallographic characterization of human cytoglobin.

de Sanctis D; Dewilde S; Pesce AAscenzi PBurmester THankeln TMoens LBolognesi M

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New insight into the haemoglobin superfamily: preliminary crystallographic characterization of human cytoglobin.

机译：新的见解血红蛋白总科:的初步晶体特性人类cytoglobin。

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Human cytoglobin, present in almost all tissue types, is a newly identified member of the Hb superfamily. A double mutant, having both cysteines replaced by serines, has been overexpressed in Escherichia coli, purified and crystallized. A highly redundant SAD data set has been collected at the haem Fe-atom absorption edge (lambda = 1.720 A) to 2.60 A resolution. The crystals belong to the orthorhombic P2(1)2(1)2(1) space group, with unit-cell parameters a = 46.8, b = 73.1, c = 98.9 A and two molecules per asymmetric unit. The anomalous difference Patterson map clearly reveals the position of the haem Fe-atom sites, thus paving the way for SAD structure determination.

机译：人类cytoglobin,出现在几乎所有的组织类型,是一种新发现的乙肝的成员总科。半胱氨酸丝氨酸所取代,在大肠杆菌中,纯化和结晶。收集在血红素Fe-atom吸收边缘(λ= 1.720)2.60决议。晶体属斜方晶系的P2 (1) 2 (1) 2 (1)空间群,晶胞参数= 46.8,b = 73.1, c = 98.9和两个分子不对称单元。帕特森显然地图显示的位置血红素Fe-atom网站,从而为悲伤铺平了道路结构的决心。

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《Acta crystallographica.Section D. Biological crystallography》 |2003年第7期|1285-1287|共3页
作者
de Sanctis D; Dewilde S; Pesce AAscenzi PBurmester THankeln TMoens LBolognesi M;
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作者单位

Department of Physics - INFM and Center for Excellence in Biomedical Research, University of Genova, Via Dodecaneso 33, I-16146 Genova, Italy.;

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正文语种英语
中图分类化学;
关键词
Cysteine; Feeling of Sadness; superfamilyAtomsInsightNOT OTHERWISE SPECIFIEDHemeHemoglobins;

机译：半胱氨酸;的感觉悲伤;superfamilyAtomsInsightNOT否则SPECIFIEDHemeHemoglobins;

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New insight into the haemoglobin superfamily: preliminary crystallographic characterization of human cytoglobin.

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