Crystallization and preliminary X-ray diffraction analysis of the red fluorescent protein eqFP611.

Nienhaus K; Vallone B; Renzi FWiedenmann JNienhaus GU

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray diffraction analysis of the red fluorescent protein eqFP611.

【24h】

Crystallization and preliminary X-ray diffraction analysis of the red fluorescent protein eqFP611.

机译：结晶和初步的x射线衍射红色荧光蛋白eqFP611分析。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

A novel red fluorescent protein, eqFP611, from the sea anemone Entacmaea quadricolor has been cloned in Escherichia coli. With excitation and emission maxima at 559 and 611 nm, this protein shows the most red-shifted emission and the largest Stokes shift of all non-modified proteins in the green fluorescent protein (GFP) family. The protein fluoresces over a wide pH range (4-10) with high quantum yield (0.45). Its photophysical properties make eqFP611 an excellent marker protein for in vivo labeling in eukaryotic systems as was shown by expression in a mammalian cell culture. eqFP611 has been crystallized in space group P6(5)22, with unit-cell parameters a = b = 77.26, c = 329.49 A. The unit cell contains 12 asymmetric units, with two monomers in each. A molecular-replacement solution has been obtained using the 48.4% homologous red fluorescent protein from Discosoma coral (DsRed).

机译：一种新型红色荧光蛋白,eqFP611,从海葵Entacmaea quadricolor被克隆在大肠杆菌。最大值在559和611海里,这种蛋白质显示了最大最红移辐射和斯托克斯所有非转基因蛋白质的绿色转变荧光蛋白(GFP)的家庭。会发出荧光在pH值范围宽(十)高量子产率(0.45)。属性使eqFP611一个优秀的标志蛋白在真核生物体内标记系统在哺乳动物表达所示细胞培养。空间群P6(5) 22日,晶胞参数A = b = 77.26, c = 329.49。12不对称单位,有两个单体。molecular-replacement解决方案获得了使用48.4%同源红色荧光蛋白质从Discosoma珊瑚(域)。

著录项

来源
《Acta crystallographica.Section D. Biological crystallography》 |2003年第7期|1253-1255|共3页
作者
Nienhaus K; Vallone B; Renzi FWiedenmann JNienhaus GU;
展开▼
作者单位

Department of Biophysics, University of Ulm, 89069 Ulm, Germany.;

展开▼
收录信息
原文格式 PDF
正文语种英语
中图分类化学;
关键词
red fluorescent protein; Red shift; Green Fluorescent ProteinsX-ray diffraction analysisX-raysCrystallization;

机译：红色荧光蛋白,红移;绿色荧光ProteinsX-ray衍射analysisX-raysCrystallization;

相似文献

外文文献
中文文献
专利

1. Science Letters:Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii [J] . GAO Yong-gui, YAO Min, TANAKA Isao 浙江大学学报（英文版）（B辑：生物医学和生物技术） . 2005,第006期
2. Red fluorescent protein (DsRed2), an ideal reporter for cotton genetic transformation and molecular breeding [J] . Lin Sun, Shuangxia Jin, Muna Alariqi, 作物学报（英文版） . 2018,第004期
3. Data mining of Arabidopsis thaliana salt-response proteins based on bioinformatics analysis Guo Meili, Gao Weixi, Yu Xuejuan, Zhou Chunxi, Liu Fujun, Liu Xin Abstract [J] . Plant Omics . 2012,第1期

机译：Data mining of Arabidopsis thaliana salt-response proteins based on bioinformatics analysis Guo Meili, Gao Weixi, Yu Xuejuan, Zhou Chunxi, Liu Fujun, Liu Xin Abstract
4. Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of human coronavirus OC43 nucleocapsid protein [O] . Chen I.J., Chou C.C., Liu C.L., 2014

机译：Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of human coronavirus OC43 nucleocapsid protein
5. De infrarood-spektrometrische analyse van gassen welke ontstaan bij de vacuumstabili-teitstest van kruiten en springstoffen [R] . H. L. Barreveld 1976

机译：来自red-spektrometrische van gassen analysis welke ontstaan bij de vacuumstabili-teitstest van kruiten en springstoffen

Crystallization and preliminary X-ray diffraction analysis of the red fluorescent protein eqFP611.

摘要

著录项

相似文献

相关主题

期刊订阅