...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2.
【24h】

Purification, crystallization and preliminary X-ray analysis of immunogenic virus-like particles formed by infectious bursal disease virus (IBDV) structural protein VP2.

机译：净化、结晶和初步的免疫原性病毒样的x射线分析粒子由传染性法氏囊病病毒(IBDV)结构蛋白VP2。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Infectious bursal disease virus (IBDV) causes a highly contagious disease in young chicks and leads to significant economic losses in the poultry industry. VP2 protein, which consists of 452 amino-acid residues, is the primary immunogen of IBDV and contains the epitopes responsible for eliciting neutralizing antibodies. When the chimeric VP2 protein (rVP2H) of a local IBDV strain P3009 was expressed alone using the baculovirus system, virus-like particles of approximately 23 nm in diameter formed spontaneously. Highly pure rVP2H particles, obtained using ammonium sulfate precipitation, immobilized metal-ion affinity chromatography and gel-filtration chromatography, were successfully crystallized using the vapour-diffusion method. These crystals, with a maximum dimension of 0.4 mm, diffracted X-rays to 4.5 A resolution, but data were only collected to 6 A. Preliminary analysis of the diffraction data showed that the rVP2H crystals belong to the cubic space group P2(1)3, with unit-cell parameter 323.1 A.The icosahedral symmetry of the particles is clearly seen in the self-rotation function maps, with dyads and triads coincident with the crystallographic axes. Each asymmetric unit contains 1/3 of the particle, or 20 rVP2H subunits, and there are four particles in a unit cell, probably in a tetrahedral arrangement.
机译：传染性法氏囊病病毒(IBDV)引起在年轻的小鸡和高度传染性疾病导致重大经济损失家禽行业。452个氨基酸残基,是主要的免疫原IBDV和含有抗原表位负责诱发中和抗体。当地IBDV嵌合VP2蛋白(rVP2H)应变P3009表达单独使用杆状病毒系统,病毒样颗粒直径大约23海里形成自发。获得使用硫酸铵沉淀,固定化金属离子亲和色谱法凝胶过滤色谱法,成功使用vapour-diffusion结晶的方法。这些晶体,最大尺寸为0.44.5毫米,x射线衍射分辨率,但是数据只收集到6。分析表明,衍射数据rVP2H晶体属于立方空间群P2(1) 3,晶胞参数323.1。显然是二十面体对称的粒子的固有转动函数映射,二分体和三合会重合晶体的轴。包含1/3的粒子,或20 rVP2H子单元,在单元有四个粒子细胞,可能在一个四面体的安排。

著录项

相似文献

  • 外文文献
  • 中文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号