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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary crystallographic study of the outer-membrane lipoprotein receptor LolB, a member of the lipoprotein localization factors.
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Crystallization and preliminary crystallographic study of the outer-membrane lipoprotein receptor LolB, a member of the lipoprotein localization factors.

机译：结晶和初步的晶体外膜脂蛋白受体的研究LolB,脂蛋白本地化的一员的因素。

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摘要

The Lol system mediates the translocation of the water-insoluble outer-membrane lipoprotein across the periplasm of Gram-negative bacteria depending on the sorting signal. The outer-membrane lipoprotein receptor LolB (21.2 kDa) is a member of the Lol system. A soluble mutant of LolB (mLolB) from Escherichia coli was crystallized in two forms. Monoclinic crystals diffract X-rays to 1.9 A resolution and belong to space group P2(1), with unit-cell parameters a = 37.2, b = 112.4, c = 47.8 A, beta = 111.4 degrees. The V(M) value is most likely to be 2.2 A(3) Da(-1), assuming the presence of two molecules in the asymmetric unit. Hexagonal crystals diffract X-rays to 2.2 A resolution and belong to space group P6(3)22, with unit-cell parameters a = b = 71.4, c = 133.9 A. The V(M) value is determined as 2.3 A(3) Da(-1), assuming a single molecule in the asymmetric unit. A four-wavelength data set was collected from a monoclinic crystal of selenomethionylated mLolB in order to perform MAD phasing. The quality ofthe initial electron-density map was sufficient to build a molecular model.
机译：Lol系统介导的易位不溶于水的外膜脂蛋白在革兰氏阴性细菌的周质不同在排序的信号。脂蛋白受体LolB (21.2 kDa)是一个成员Lol系统。从大肠杆菌中结晶(mLolB)两种形式。1.9分辨率和属于空间群P2 (1),晶胞参数= 37.2,= 112.4 b, c= 47.8,β= 111.4度。最有可能是(3)达2.2(1),假设存在两个分子的不对称单元。2.2六角晶体衍射x射线分辨率和属于空间群P6(3) 22日晶胞参数a = b = 71.4, c = 133.9答:V (M)值被确定为2.3 (3)Da(1),假设中的一个分子不对称单元。收集的单斜晶体selenomethionylated mLolB为了执行疯了定相。电子密度足以建立一个地图分子模型。

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