Choice of data-collection parameters based on statistic modelling.

Popov AN; Bourenkov GP

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Choice of data-collection parameters based on statistic modelling.

机译：基于收集的选择参数统计模型。

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A new method and the software program BEST for optimal planning of X-ray data collection from protein crystals using the rotation method are presented. From one or a few initial diffraction images, BEST estimates the statistical characteristics of the data set for different combinations of data-collection parameters and suggests the most optimal ones. The anisotropy in diffraction and the permitted width of oscillation without spatially overlapping reflections are taken into account. According to the option chosen, the optimal set of parameters provides a given average signal-to-noise ratio at a given resolution either in the shortest time or with the minimum total radiation dose. BEST has been successfully used at the protein crystallography beamlines at DORIS (DESY). The software proved to be extremely useful in using the available data-collection time in the most efficient way.

机译：最好的一种新方法和软件程序最优规划的x射线数据收集蛋白质晶体使用旋转的方法提出了。图片,最好的估计,统计不同的数据集的特征数据收集的参数和组合表明最优的。衍射和允许的宽度振荡没有空间重叠反思是考虑。所选择的选项,最优的一组参数提供了一个给定的平均信噪比在最短的时间内或一个给定的决议最低的总辐射剂量。被成功地用于蛋白质晶体学beamlines多丽丝(谜底)。软件在使用被证明是非常有用的在最可用的数据收集时间有效的方式。

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《Acta crystallographica.Section D. Biological crystallography》 |2003年第7期|1145-1153|共9页
作者
Popov AN; Bourenkov GP;
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作者单位

European Molecular Biology Laboratory (EMBL) Hamburg Outstation, c/o DESY, Notkestrasse 85, 22603 Hamburg, Germany. sasha@embl-hamburg.de;

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原文格式 PDF
正文语种英语
中图分类化学;
关键词
statistical models (nuclear); X-ray data; beamline;

机译：统计模型(核);x射线数据;beamline;

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Choice of data-collection parameters based on statistic modelling.

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