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首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons.
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Apocrustacyanin C(1) crystals grown in space and on earth using vapour-diffusion geometry: protein structure refinements and electron-density map comparisons.

机译：Apocrustacyanin C(1)晶体生长在空间和地球上使用vapour-diffusion几何:蛋白质结构改进和电子密度图比较。

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摘要

Models of apocrustacyanin C(1) were refined against X-ray data recorded on Bending Magnet 14 at the ESRF to resolutions of 1.85 and 2 A from a space-grown and an earth-grown crystal, respectively, both using vapour-diffusion crystal-growth geometry. The space crystals were grown in the APCF on the NASA Space Shuttle. The microgravity crystal growth showed a cyclic nature attributed to Marangoni convection, thus reducing the benefits of the microgravity environment, as reported previously [Chayen et al. (1996), Q. Rev. Biophys. 29, 227-278]. A subsequent mosaicity evaluation, also reported previously, showed only a partial improvement in the space-grown crystals over the earth-grown crystals [Snell et al. (1997), Acta Cryst. D53, 231-239], contrary to the case for lysozyme crystals grown in space with liquid-liquid diffusion, i.e. without any major motion during growth [Snell et al. (1995), Acta Cryst. D52, 1099-1102]. In this paper, apocrustacyanin C(1) electron-density maps from the two refined modelsare now compared. It is concluded that the electron-density maps of the protein and the bound waters are found to be better overall for the structures of apocrustacyanin C(1) studied from the space-grown crystal compared with those from the earth-grown crystal, even though both crystals were grown using vapour-diffusion crystal-growth geometry. The improved residues are on the surface of the protein, with two involved in or nearby crystal lattice-forming interactions, thus linking an improved crystal-growth mechanism to the molecular level. The structural comparison procedures developed should themselves be valuable for evaluating crystal-growth procedures in the future.
机译：模型apocrustacyanin C(1)精制对x射线数据记录在弯曲磁铁14在ESRF决议的1.85和2空间长大和大麦的水晶,分别使用vapour-diffusion两种晶体生长几何学。生长在APCF NASA航天飞机。微重力晶体生长循环自然归因于马朗戈尼对流,因此减少微重力的好处环境,先前报道[Chayen等。(1996),问:启Biophys。29日,227 - 278]。随后mosaicity评价,也报道以前,只显示部分改善在大麦空间长大晶体斯奈尔晶体(et al .(1997),《结晶。231 - 239年),与溶菌酶晶体生长与液-液空间扩散,即没有任何主要的运动中斯奈尔增长(et al .(1995),《结晶。1099 - 1102年)。电子密度图的两个精炼现在modelsare相比。电子密度的蛋白质和地图绑定水域发现更好的整体apocrustacyanin C(1)的结构进行了研究从空间长大水晶相比大麦的水晶,尽管两种使用vapour-diffusion晶体生长晶体生长几何学。表面的蛋白质,两个参与或附近水晶lattice-forming交互,从而将一种改进的晶体生长机制,分子水平上。结构比较程序开发自己应该有价值的评价吗晶体生长过程。

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