Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa.

Yamasaki M; Moriwaki S; Hashimoto WMikami BMurata K

首页> 外文期刊>Acta crystallographica.Section D. Biological crystallography >Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa.

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Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa.

机译：结晶和初步的x射线分析褐藻胶裂解酶,PL-7家族的一员,从铜绿假单胞菌。

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Alginate lyase depolymerizes alginate, a heteropolysaccharide consisting of alpha-L-guluronate and beta-D-mannuronate, through a beta-elimination reaction. A protein PA1167 with a molecular mass of 25 kDa produced by Pseudomonas aeruginosa is an alginate lyase classified into polysaccharide lyase family PL-7. The enzyme was crystallized at 293 K in a drop solution comprising 1.4 M sodium chloride, 0.1 M potassium sodium phosphate and 0.1 M 2-morpholinoethanesulfonate-sodium hydroxide pH 6.5 by means of the vapor-diffusion method. The crystals were monoclinic and belonged to space group P2(1), with unit-cell parameters a = 43.4, b = 70.3, c = 67.4 A, beta = 94.5 degrees. Diffraction data were collected to 2.0 A from a single crystal.

机译：海藻酸褐藻胶裂解酶解聚,杂多糖组成的alpha-L-guluronate beta-D-mannuronate,通过beta-elimination反应。PA1167 25 kDa的分子质量铜绿假单胞菌是一种海藻酸裂解酶分为家庭PL-7多糖裂合酶。这种酶被固定在293 K下降解决方案包括1.4 M氯化钠,0.1 M磷酸钾钠和0.1米氢氧化2-morpholinoethanesulfonate-sodium pH值6.5通过扩散的方法。是单斜晶体,属于空间集团P2(1),晶胞参数= 43.4,b = 70.3, c = 67.4,β= 94.5度。2.0从衍射数据收集单晶。

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《Acta crystallographica.Section D. Biological crystallography》 |2003年第8期|1499-1501|共3页
作者
Yamasaki M; Moriwaki S; Hashimoto WMikami BMurata K;
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Division of Agronomy and Horticultural Science, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.;

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正文语种英语
中图分类化学;
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poly(beta-D-mannuronate) lyase; X-ray analysis; Pseudomonas aeruginosaDrop Solution Dosage FormfamilyCrystallization;

机译：保利(beta-D-mannuronate)裂合酶;x射线分析;假单胞菌aeruginosaDrop解决方案剂量FormfamilyCrystallization;

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Crystallization and preliminary X-ray analysis of alginate lyase, a member of family PL-7, from Pseudomonas aeruginosa.

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